WEKO3
アイテム
{"_buckets": {"deposit": "c1aee68e-61cf-41ef-94df-09a26b40b3f6"}, "_deposit": {"created_by": 1, "id": "83841", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "83841"}, "status": "published"}, "_oai": {"id": "oai:repo.qst.go.jp:00083841", "sets": ["1"]}, "author_link": ["1011495", "1011493", "1011492", "1011491", "1011494", "1011496", "1011497"], "item_8_biblio_info_7": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2021-02", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "5", "bibliographicPageStart": "119021", "bibliographicVolumeNumber": "246", "bibliographic_titles": [{"bibliographic_title": "Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy"}]}]}, "item_8_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "In this study, a facile, sensitive, and precise lab-on-a-chip electrophoretic method coupled with light-emitting diode induced fluorescence (LED-IF) detection was developed to assay three antiepileptic drugs, namely, vigabatrin, pregabalin, and gabapentin, in pharmaceutical formulations. The analytes were derivatised offline for the first time with fluorescine-5-isothiocyanate (FITC) to yield highly fluorescent derivatives with λex/em of 490/520 nm. The FITC-labelled analytes were injected, separated, and quantitated by a microfluidic electrophoresis device using fluorescence detection. The labelled analytes were monitored using a blue LED-IF system. The separation conditions were significantly optimised adding specific concentrations of heptakis-(2,6-di-O-methyl)-β-cyclodextrin (HDM-β-CD) and methylcellulose to the buffer solution (40 mM borate buffer). HDM-β-CD acted as a selective host for the studied antiepileptic drugs, rendering a high separation efficiency. Methylcellulose was used as an efficient dynamic coating polymer to prevent the labelled drugs from being adsorbed on the inner surfaces of the poly (methylmethacrylate) microchannels. A laboratory-prepared ternary mixture of the three antiepileptic drugs was separated within 100 s with acceptable resolution between all the three analytes (Rs \u003e 3) and a high number of theoretical plates (N) for each analyte (N ≈ 106 plates/m). The sensitivity of the method was enhanced approximately 80-fold by stacking to yield a detection limit below 0.6 ng mL−1 in the concentration range of 2.0–200.0 ng mL−1. The method was successfully validated for analysing the studied drugs in their pharmaceutical formulations.", "subitem_description_type": "Abstract"}]}, "item_8_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "10.1016/j.saa.2020.119021", "subitem_relation_type_select": "DOI"}}]}, "item_8_relation_17": {"attribute_name": "関連サイト", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "https://www.sciencedirect.com/science/article/abs/pii/S1386142520310003?via%3Dihub#!", "subitem_relation_type_select": "URI"}}]}, "item_8_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1386-1425", "subitem_source_identifier_type": "ISSN"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "metadata only access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_14cb"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "M.Zeid, Abdallah"}], "nameIdentifiers": [{"nameIdentifier": "1011491", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Jeehan M. Nasr, Jenny"}], "nameIdentifiers": [{"nameIdentifier": "1011492", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Belal, Fathalla"}], "nameIdentifiers": [{"nameIdentifier": "1011493", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "I. Walash, Mohamed"}], "nameIdentifiers": [{"nameIdentifier": "1011494", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yoshinobu, Baba"}], "nameIdentifiers": [{"nameIdentifier": "1011495", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kaji, Noritada"}], "nameIdentifiers": [{"nameIdentifier": "1011496", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yoshinobu, Baba", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "1011497", "nameIdentifierScheme": "WEKO"}]}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Determination of three antiepileptic drugs in pharmaceutical formulations using microfluidic chips coupled with light-emitting diode induced fluorescence detection", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Determination of three antiepileptic drugs in pharmaceutical formulations using microfluidic chips coupled with light-emitting diode induced fluorescence detection"}]}, "item_type_id": "8", "owner": "1", "path": ["1"], "permalink_uri": "https://repo.qst.go.jp/records/83841", "pubdate": {"attribute_name": "公開日", "attribute_value": "2021-09-29"}, "publish_date": "2021-09-29", "publish_status": "0", "recid": "83841", "relation": {}, "relation_version_is_last": true, "title": ["Determination of three antiepileptic drugs in pharmaceutical formulations using microfluidic chips coupled with light-emitting diode induced fluorescence detection"], "weko_shared_id": -1}
Determination of three antiepileptic drugs in pharmaceutical formulations using microfluidic chips coupled with light-emitting diode induced fluorescence detection
https://repo.qst.go.jp/records/83841
https://repo.qst.go.jp/records/838414d593aca-6358-4704-9e6d-0d625bf433bd
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2021-09-29 | |||||
タイトル | ||||||
タイトル | Determination of three antiepileptic drugs in pharmaceutical formulations using microfluidic chips coupled with light-emitting diode induced fluorescence detection | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
著者 |
M.Zeid, Abdallah
× M.Zeid, Abdallah× Jeehan M. Nasr, Jenny× Belal, Fathalla× I. Walash, Mohamed× Yoshinobu, Baba× Kaji, Noritada× Yoshinobu, Baba |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | In this study, a facile, sensitive, and precise lab-on-a-chip electrophoretic method coupled with light-emitting diode induced fluorescence (LED-IF) detection was developed to assay three antiepileptic drugs, namely, vigabatrin, pregabalin, and gabapentin, in pharmaceutical formulations. The analytes were derivatised offline for the first time with fluorescine-5-isothiocyanate (FITC) to yield highly fluorescent derivatives with λex/em of 490/520 nm. The FITC-labelled analytes were injected, separated, and quantitated by a microfluidic electrophoresis device using fluorescence detection. The labelled analytes were monitored using a blue LED-IF system. The separation conditions were significantly optimised adding specific concentrations of heptakis-(2,6-di-O-methyl)-β-cyclodextrin (HDM-β-CD) and methylcellulose to the buffer solution (40 mM borate buffer). HDM-β-CD acted as a selective host for the studied antiepileptic drugs, rendering a high separation efficiency. Methylcellulose was used as an efficient dynamic coating polymer to prevent the labelled drugs from being adsorbed on the inner surfaces of the poly (methylmethacrylate) microchannels. A laboratory-prepared ternary mixture of the three antiepileptic drugs was separated within 100 s with acceptable resolution between all the three analytes (Rs > 3) and a high number of theoretical plates (N) for each analyte (N ≈ 106 plates/m). The sensitivity of the method was enhanced approximately 80-fold by stacking to yield a detection limit below 0.6 ng mL−1 in the concentration range of 2.0–200.0 ng mL−1. The method was successfully validated for analysing the studied drugs in their pharmaceutical formulations. | |||||
書誌情報 |
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 巻 246, 号 5, p. 119021, 発行日 2021-02 |
|||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1386-1425 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.saa.2020.119021 | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://www.sciencedirect.com/science/article/abs/pii/S1386142520310003?via%3Dihub#! |