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Neutron diffraction studies of [NiFe]-hydrogenase from Desulfovibrio vulgaris Miyazaki F
https://repo.qst.go.jp/records/80219
https://repo.qst.go.jp/records/8021923a47dc7-1045-4ddf-b8af-cfd2fb2c489b
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2020-07-16 | |||||
| タイトル | ||||||
| タイトル | Neutron diffraction studies of [NiFe]-hydrogenase from Desulfovibrio vulgaris Miyazaki F | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Tamada, Taro
× Tamada, Taro× Hiromoto, Takeshi× Nishikawa, Koji× Hirano, Yuu× Kusaka, Katsuhiro× Coates, Leighton× Higuchi, Yoshiki× Tamada, Taro× Hiromoto, Takeshi× Hirano, Yuu |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | [NiFe]-hydrogenase contains several metal centers, including the bimetallic Ni-Fe active site, iron-sulfur clusters and an Mg2+ center. X-ray structure analysis of the [NiFe]-hydrogenase from Desulfovibrio vulgaris Miyazaki F elucidated that Ni is coordinated by four sulfur atoms of cysteinyl residues, and two of them coordinate Fe making a bridge between Ni and Fe. The third bridging ligand between Ni and Fe presents depending on the oxidation states. In the oxidized (inactive) form, the third bridging ligand is assigned as an oxygen species from X-ray structure analysis. In the reduced (active) form, single crystal EPR analyses showed that a hydride binds between two metal atoms. Recently, a hydride between Ni and Fe has been reported by subatomic resolution X-ray structure analysis. However, the assignment of a hydride by X-ray crystallography is still controversial, and proton transfer pathways in the enzyme are still unclear. We aim to collect neutron diffraction data of the [NiFe]-hydrogenase in both oxidized and reduced forms to obtain precise information of the bridging ligand species between Ni and Fe in the reduced form and the proton transfer pathways. We have already succeeded in preparation of large crystals (>1 mm3) in both forms. Neutron diffraction experiments were carried out under cryogenic condition in order to preserve the reduced form of the enzyme during the data collection at MLF/J-PARC and SNS/ORNL. We could observe diffraction spots up to 2.0 and 1.9 Å resolution from a crystal in the oxidized and the reduced forms, respectively. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | The 20th Annual Meeting of the Protein Science Society of Japan | |||||
| 発表年月日 | ||||||
| 日付 | 2020-07-15 | |||||
| 日付タイプ | Issued | |||||
