WEKO3
アイテム
{"_buckets": {"deposit": "174115a7-d479-4c53-8051-39a572c6aaf2"}, "_deposit": {"created_by": 1, "id": "79969", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "79969"}, "status": "published"}, "_oai": {"id": "oai:repo.qst.go.jp:00079969", "sets": ["1"]}, "author_link": ["870821", "870826", "870825", "870814", "870824", "870820", "870832", "870819", "870828", "870816", "870830", "870817", "870813", "870831", "870829", "870822", "870833", "870834", "870815", "870818", "870823", "870827"], "item_8_biblio_info_7": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2020-05", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "20", "bibliographicPageEnd": "10824", "bibliographicPageStart": "10818", "bibliographicVolumeNumber": "117", "bibliographic_titles": [{"bibliographic_title": "Proceedings of the National Academy of Sciences of United States of America "}]}]}, "item_8_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Recent advances in neutron crystallographic studies have provided structural bases for quantum behaviors of protons observed in enzymatic reactions. Thus, we resolved the neutron crystal structure of a bacterial copper amine oxidase, which contains a prosthetic copper ion and a protein-derived redox cofactor, topa quinone. We solved hitherto unknown structures of the active site, including a keto/enolate equilibrium of the cofactor with a non-planar quinone ring, unusual proton sharing between the cofactor and the catalytic base, and metal-induced deprotonation of a histidine residue that coordinates to the copper. Our findings show a refined active-site structure that gives detailed information on the protonation state of dissociable groups, such as the quinone cofactor, which are critical for catalytic reactions.", "subitem_description_type": "Abstract"}]}, "item_8_publisher_8": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "National Academy of Sciences"}]}, "item_8_relation_13": {"attribute_name": "PubMed番号", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "16576751", "subitem_relation_type_select": "PMID"}}]}, "item_8_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "10.1073/pnas.1922538117", "subitem_relation_type_select": "DOI"}}]}, "item_8_relation_17": {"attribute_name": "関連サイト", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "https://www.pnas.org/content/117/20/10818", "subitem_relation_type_select": "URI"}}]}, "item_8_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0027-8424", "subitem_source_identifier_type": "ISSN"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "metadata only access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_14cb"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Murakawa, Takeshi"}], "nameIdentifiers": [{"nameIdentifier": "870813", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kurihara, Kazuo"}], "nameIdentifiers": [{"nameIdentifier": "870814", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Shoji, Mitsuo"}], "nameIdentifiers": [{"nameIdentifier": "870815", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Shibazaki, Chie"}], "nameIdentifiers": [{"nameIdentifier": "870816", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sunami, Tomoko"}], "nameIdentifiers": [{"nameIdentifier": "870817", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Tamada, Taro"}], "nameIdentifiers": [{"nameIdentifier": "870818", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yano, Naomine"}], "nameIdentifiers": [{"nameIdentifier": "870819", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yamada, Taro"}], "nameIdentifiers": [{"nameIdentifier": "870820", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kusaka, Katsuhiro"}], "nameIdentifiers": [{"nameIdentifier": "870821", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Suzuki, Mamoru"}], "nameIdentifiers": [{"nameIdentifier": "870822", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Shigeta, Yasuteru"}], "nameIdentifiers": [{"nameIdentifier": "870823", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kuroki, Ryota"}], "nameIdentifiers": [{"nameIdentifier": "870824", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hayashi, Hideyuki"}], "nameIdentifiers": [{"nameIdentifier": "870825", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yano, Takato"}], "nameIdentifiers": [{"nameIdentifier": "870826", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Tanizawa, Katsuyuki"}], "nameIdentifiers": [{"nameIdentifier": "870827", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Adachi, Motoyasu"}], "nameIdentifiers": [{"nameIdentifier": "870828", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Okajima, Toshihide"}], "nameIdentifiers": [{"nameIdentifier": "870829", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kurihara, Kazuo", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "870830", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Shibazaki, Chie", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "870831", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sunami, Tomoko", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "870832", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Tamada, Taro", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "870833", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Adachi, Motoyasu", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "870834", "nameIdentifierScheme": "WEKO"}]}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Neutron crystallography of copper amine oxidase reveals keto/enolate interconversion of the quinone cofactor and unusual proton sharing", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Neutron crystallography of copper amine oxidase reveals keto/enolate interconversion of the quinone cofactor and unusual proton sharing"}]}, "item_type_id": "8", "owner": "1", "path": ["1"], "permalink_uri": "https://repo.qst.go.jp/records/79969", "pubdate": {"attribute_name": "公開日", "attribute_value": "2020-04-01"}, "publish_date": "2020-04-01", "publish_status": "0", "recid": "79969", "relation": {}, "relation_version_is_last": true, "title": ["Neutron crystallography of copper amine oxidase reveals keto/enolate interconversion of the quinone cofactor and unusual proton sharing"], "weko_shared_id": -1}
Neutron crystallography of copper amine oxidase reveals keto/enolate interconversion of the quinone cofactor and unusual proton sharing
https://repo.qst.go.jp/records/79969
https://repo.qst.go.jp/records/7996990fb0b67-f838-4f3d-862a-1bdf47720d59
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2020-04-01 | |||||
タイトル | ||||||
タイトル | Neutron crystallography of copper amine oxidase reveals keto/enolate interconversion of the quinone cofactor and unusual proton sharing | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
著者 |
Murakawa, Takeshi
× Murakawa, Takeshi× Kurihara, Kazuo× Shoji, Mitsuo× Shibazaki, Chie× Sunami, Tomoko× Tamada, Taro× Yano, Naomine× Yamada, Taro× Kusaka, Katsuhiro× Suzuki, Mamoru× Shigeta, Yasuteru× Kuroki, Ryota× Hayashi, Hideyuki× Yano, Takato× Tanizawa, Katsuyuki× Adachi, Motoyasu× Okajima, Toshihide× Kurihara, Kazuo× Shibazaki, Chie× Sunami, Tomoko× Tamada, Taro× Adachi, Motoyasu |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Recent advances in neutron crystallographic studies have provided structural bases for quantum behaviors of protons observed in enzymatic reactions. Thus, we resolved the neutron crystal structure of a bacterial copper amine oxidase, which contains a prosthetic copper ion and a protein-derived redox cofactor, topa quinone. We solved hitherto unknown structures of the active site, including a keto/enolate equilibrium of the cofactor with a non-planar quinone ring, unusual proton sharing between the cofactor and the catalytic base, and metal-induced deprotonation of a histidine residue that coordinates to the copper. Our findings show a refined active-site structure that gives detailed information on the protonation state of dissociable groups, such as the quinone cofactor, which are critical for catalytic reactions. | |||||
書誌情報 |
Proceedings of the National Academy of Sciences of United States of America 巻 117, 号 20, p. 10818-10824, 発行日 2020-05 |
|||||
出版者 | ||||||
出版者 | National Academy of Sciences | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0027-8424 | |||||
PubMed番号 | ||||||
識別子タイプ | PMID | |||||
関連識別子 | 16576751 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1073/pnas.1922538117 | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://www.pnas.org/content/117/20/10818 |