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Neutron crystallography of copper amine oxidase reveals keto/enolate interconversion of the quinone cofactor and unusual proton sharing
https://repo.qst.go.jp/records/79969
https://repo.qst.go.jp/records/7996990fb0b67-f838-4f3d-862a-1bdf47720d59
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2020-04-01 | |||||
| タイトル | ||||||
| タイトル | Neutron crystallography of copper amine oxidase reveals keto/enolate interconversion of the quinone cofactor and unusual proton sharing | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Murakawa, Takeshi
× Murakawa, Takeshi× Kurihara, Kazuo× Shoji, Mitsuo× Shibazaki, Chie× Sunami, Tomoko× Tamada, Taro× Yano, Naomine× Yamada, Taro× Kusaka, Katsuhiro× Suzuki, Mamoru× Shigeta, Yasuteru× Kuroki, Ryota× Hayashi, Hideyuki× Yano, Takato× Tanizawa, Katsuyuki× Adachi, Motoyasu× Okajima, Toshihide× Kurihara, Kazuo× Shibazaki, Chie× Sunami, Tomoko× Tamada, Taro× Adachi, Motoyasu |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Recent advances in neutron crystallographic studies have provided structural bases for quantum behaviors of protons observed in enzymatic reactions. Thus, we resolved the neutron crystal structure of a bacterial copper amine oxidase, which contains a prosthetic copper ion and a protein-derived redox cofactor, topa quinone. We solved hitherto unknown structures of the active site, including a keto/enolate equilibrium of the cofactor with a non-planar quinone ring, unusual proton sharing between the cofactor and the catalytic base, and metal-induced deprotonation of a histidine residue that coordinates to the copper. Our findings show a refined active-site structure that gives detailed information on the protonation state of dissociable groups, such as the quinone cofactor, which are critical for catalytic reactions. | |||||
| 書誌情報 |
Proceedings of the National Academy of Sciences of United States of America 巻 117, 号 20, p. 10818-10824, 発行日 2020-05 |
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| 出版者 | ||||||
| 出版者 | National Academy of Sciences | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0027-8424 | |||||
| PubMed番号 | ||||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | 16576751 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1073/pnas.1922538117 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://www.pnas.org/content/117/20/10818 | |||||
