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Development of the ex vivo spermatogenesis technique for advanced radiotherapeutic science
https://repo.qst.go.jp/records/72640
https://repo.qst.go.jp/records/72640bfece01a-a089-42ad-9fc0-b980e91f0054
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2018-01-16 | |||||
| タイトル | ||||||
| タイトル | Development of the ex vivo spermatogenesis technique for advanced radiotherapeutic science | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Fukunaga, Hisanori
× Fukunaga, Hisanori× Kaminaga, Kiichi× Sato, Takuya× T., Butterworth Karl× Yokoya, Akinari× Ogawa, Takehiko× 神長 輝一× 横谷 明徳 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Modern radiotherapeutic techniques can change beam shape and intensity as the machine of a radiation source moves around the patient body to match the shape of the tumor. These advancements have significantly contributed to better clinical outcomes for the tumor; however, they have also dramatically complicated the risk assessments of the surrounding normal tissues due to the heterogeneous radiation dose distribution. In this study, we develop an ex vivo mouse testis culture as a model to determine the biological effects on the germ stem cells and spermatogenesis from the exposure to such modified radiation fields. This organ culture method, using the Acr-GFP transgenic mice, is useful for observing the process of spermatogenesis because the start of meiosis can easily be detected by the GFP expression in the mice testis. The testes were obtained from 7 days postpartum mice, and then they were transferred to an X-ray irradiation facility and cultured for more than one month. We observed in real time and analyzed the radiation-induced impacts on the process of spermatogenesis. Our preliminary data validated our methodology, which accurately reproduces X-ray-induced male germ toxicity, such as temporary infertility and permanent sterility. Furthermore, we have also succeeded in detecting the change in the meiosis process induced by exposure to modified X-ray fields. These results indicate that our model is a robust approach for further investigation of radiotherapeutic toxicity and/or its side effects. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 2017年度生命科学系学会合同年次大会 | |||||
| 発表年月日 | ||||||
| 日付 | 2017-12-06 | |||||
| 日付タイプ | Issued | |||||
