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A method for imaging of uranium distribution on rat kidney sections by detection of α-particle tracks using CR-39 plastic nuclear track detectors.
https://repo.qst.go.jp/records/72555
https://repo.qst.go.jp/records/725558b4fc409-a944-4a2d-bc05-1d22c9828706
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2017-12-04 | |||||
| タイトル | ||||||
| タイトル | A method for imaging of uranium distribution on rat kidney sections by detection of α-particle tracks using CR-39 plastic nuclear track detectors. | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Konishi, Teruaki
× Konishi, Teruaki× Kodaira, Satoshi× Itakura, Yuichi× Ohsawa, Daisuke× Homma-Takeda, Shino× 小西 輝昭× 小平 聡× 板倉 雄一× 大澤 大輔× 武田 志乃 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Uranium (U) is known as a global contaminant and is a major concern for health risk due to its high toxicity. In this study, our aim was to develop a possible simple method to identify accumulated regions or localized sites of U in kidney using CR-39 plastic nuclear track detector. To demonstrate our method, the renal cryo-sections (5 μm-t) from Wistar male rats (10 weeks old) administered with U-acetate (2 mg/kg) subcutaneously were prepared on day one after administration. The section were placed on CR-39, stored for one year and then etched in a 7 M NaOH solution at 70 ° C for 3 hours to visualize the α-tracks. Addition to the detection of α-tracks, the difference of UV light absorption of tissue structure affected the bulk etch velocity resulted surface roughness of CR-39 that represented tissue structure. As a result, total of 2449 α-tracks were detected in analyzed area of 144 mm2. The cortex (CO), the outer stripe of the outer medulla (OSOM), and the medulla were measured to be 77.2 mm2, 39.4 mm2, 22.7 mm2 from the HE-stained image and were identical with the transferred tissue relief on the CR-39. Number of α-tracks detected in CO and OSOM were 345 and 1986, indicating that the accumulation of U was nearly 6-fold higher in OSOM than CO. As conclusion, this method made possible to simultaneously identify the U accumulation region in the tissue section depending on the trajectory of α-tracks. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Micros 2017 - 17th International Symposium on Microdosimetry | |||||
| 発表年月日 | ||||||
| 日付 | 2017-11-07 | |||||
| 日付タイプ | Issued | |||||
