WEKO3
アイテム
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Irradiation alters the invasive potential of PANC-1 cell line thorough NO and NOS-PI3K-AKT pathway activities in addition to the SerP activity
https://repo.qst.go.jp/records/71061
https://repo.qst.go.jp/records/71061f7dfe84f-5b7f-4bb9-b6c4-3806a6743e65
Item type | 会議発表用資料 / Presentation(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2013-03-08 | |||||
タイトル | ||||||
タイトル | Irradiation alters the invasive potential of PANC-1 cell line thorough NO and NOS-PI3K-AKT pathway activities in addition to the SerP activity | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
資源タイプ | conference object | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
著者 |
Fujita, Mayumi
× Fujita, Mayumi× Yamada, Shigeru× Imai, Takashi× 藤田 真由美× 山田 滋× 今井 高志 |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | We have previously reported that Carbon ion (C-ion) irradiation suppresses the invasiveness of several pancreatic cancer cell lines, MIAPaCa-2, BxPC-3, and AsPC-1 cells, however, we also observed that C-ion irradiation enhanced invasion in PANC-1 cells, which occurred via the activation of serine proteases (SerP), plasmin, and uPA. Treatment of C-ion irradiated PANC-1 with SerP inhibitor and ROCK inhibitor resulted in reduced invasiveness, but only by 50%, suggesting that there are more factors that have a role in C-ion-irradiation-induced PANC-1 invasion. To study the molecular factors involved in irradiation-induced PANC-1 invasion, we used the Screening Committee of Anticancer Drugs (SCADS) compound library and found that nitric oxide synthase (NOS) inhibitors and phosphatidylinositol 3-kinases (PI3K) inhibitors were both effective in reducing PANC-1 invasion. Most invaded PANC-1 cells were nitric oxide (NO)-producing cells, and the NOS-PI3K-AKT pathway was activated in these cells. This effect could be abrogated by NOS inhibitor treatment, which also reduced invasion. The population of NO-producing cells was increased by C-ion irradiation, thereby enhancing C-ion-irradiation-induced PANC-1 invasion. These results suggested that irradiation alters the invasive potential through NO and NOS-PI3K-AKT pathway activities in addition to the SerP activity. | |||||
会議概要(会議名, 開催地, 会期, 主催者等) | ||||||
内容記述タイプ | Other | |||||
内容記述 | Ninth AACR-Japanese Cancer Association Joint Conference | |||||
発表年月日 | ||||||
日付 | 2013-02-25 | |||||
日付タイプ | Issued |