WEKO3
アイテム
{"_buckets": {"deposit": "819a18b7-1387-4ae7-992f-5c9e8b85e015"}, "_deposit": {"created_by": 1, "id": "68897", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "68897"}, "status": "published"}, "_oai": {"id": "oai:repo.qst.go.jp:00068897", "sets": ["28"]}, "author_link": ["676199", "676201", "676200", "676198", "676196", "676197"], "item_10005_date_7": {"attribute_name": "発表年月日", "attribute_value_mlt": [{"subitem_date_issued_datetime": "2007-03-26", "subitem_date_issued_type": "Issued"}]}, "item_10005_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Objectives: In cancer gene therapy, restriction of antitumor transgene expression in a radiation field by use of ionizing radiation-inducible promoters is one of the promising approaches for tumor-specific gene delivery. Although tumor suppressor protein p53 is induced by low doses (\u003c1 Gy) of radiation, there have been only a few reports indicating potential utilization of a p53-target gene promoter, such as that of the p21 gene. This is mainly because the transiently transfected promoter of p53-target genes is not much sensitive to radiation. The purpose of this study is to evaluate radiation sensitivity of the p53-target gene promoter integrated into host cell chromosomes by use of adeno-associated virus (rAAV) vectors.\n\\nMethods: The response of the p21 gene promoter to low-dose radiation was tested when transduced into a human breast cancer cell line MCF-7 by use of rAAV vectors. The suicide gene HSVtk linked to the p21 gene promoter was transduced into MCF-7 cells by rAAV, and their radiation-sensitivity was examined. \n\\nResults: It was shown that the p21 gene promoter transduced by rAAV vectors was more highly radiation-responsive than that transiently transfected by electroporation. A significant induction of the p21 gene promoter by radiation of low doses down to 0.2 Gy was observed. When cells were transduced with the p21 gene promoter-driven HSVtk gene by rAAV vector, they were siginificantly sensitized to repetitive treatment with low dose radiation (1 Gy) in the presence of the prodrug ganciclovir.", "subitem_description_type": "Abstract"}]}, "item_10005_description_6": {"attribute_name": "会議概要(会議名, 開催地, 会期, 主催者等)", "attribute_value_mlt": [{"subitem_description": "2nd International Congress of Molecular Medicine", "subitem_description_type": "Other"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "metadata only access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_14cb"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Nenoi, Mitsuru"}], "nameIdentifiers": [{"nameIdentifier": "676196", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nakajima, Tetsuo"}], "nameIdentifiers": [{"nameIdentifier": "676197", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Taki, Keiko"}], "nameIdentifiers": [{"nameIdentifier": "676198", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "根井 充", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "676199", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "中島 徹夫", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "676200", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "瀧 景子", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "676201", "nameIdentifierScheme": "WEKO"}]}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "conference object", "resourceuri": "http://purl.org/coar/resource_type/c_c94f"}]}, "item_title": "Low dose radiation inducible vectors for cancer gene therapy", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Low dose radiation inducible vectors for cancer gene therapy"}]}, "item_type_id": "10005", "owner": "1", "path": ["28"], "permalink_uri": "https://repo.qst.go.jp/records/68897", "pubdate": {"attribute_name": "公開日", "attribute_value": "2007-03-30"}, "publish_date": "2007-03-30", "publish_status": "0", "recid": "68897", "relation": {}, "relation_version_is_last": true, "title": ["Low dose radiation inducible vectors for cancer gene therapy"], "weko_shared_id": -1}
Low dose radiation inducible vectors for cancer gene therapy
https://repo.qst.go.jp/records/68897
https://repo.qst.go.jp/records/68897213164eb-435a-4f20-8f78-303a15a80006
Item type | 会議発表用資料 / Presentation(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2007-03-30 | |||||
タイトル | ||||||
タイトル | Low dose radiation inducible vectors for cancer gene therapy | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
資源タイプ | conference object | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
著者 |
Nenoi, Mitsuru
× Nenoi, Mitsuru× Nakajima, Tetsuo× Taki, Keiko× 根井 充× 中島 徹夫× 瀧 景子 |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Objectives: In cancer gene therapy, restriction of antitumor transgene expression in a radiation field by use of ionizing radiation-inducible promoters is one of the promising approaches for tumor-specific gene delivery. Although tumor suppressor protein p53 is induced by low doses (<1 Gy) of radiation, there have been only a few reports indicating potential utilization of a p53-target gene promoter, such as that of the p21 gene. This is mainly because the transiently transfected promoter of p53-target genes is not much sensitive to radiation. The purpose of this study is to evaluate radiation sensitivity of the p53-target gene promoter integrated into host cell chromosomes by use of adeno-associated virus (rAAV) vectors. \nMethods: The response of the p21 gene promoter to low-dose radiation was tested when transduced into a human breast cancer cell line MCF-7 by use of rAAV vectors. The suicide gene HSVtk linked to the p21 gene promoter was transduced into MCF-7 cells by rAAV, and their radiation-sensitivity was examined. \nResults: It was shown that the p21 gene promoter transduced by rAAV vectors was more highly radiation-responsive than that transiently transfected by electroporation. A significant induction of the p21 gene promoter by radiation of low doses down to 0.2 Gy was observed. When cells were transduced with the p21 gene promoter-driven HSVtk gene by rAAV vector, they were siginificantly sensitized to repetitive treatment with low dose radiation (1 Gy) in the presence of the prodrug ganciclovir. |
|||||
会議概要(会議名, 開催地, 会期, 主催者等) | ||||||
内容記述タイプ | Other | |||||
内容記述 | 2nd International Congress of Molecular Medicine | |||||
発表年月日 | ||||||
日付 | 2007-03-26 | |||||
日付タイプ | Issued |