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Radio-sensitivity of the LEC rat
https://repo.qst.go.jp/records/67429
https://repo.qst.go.jp/records/674299f8b1606-df02-47c5-91ec-747065b8e007
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2003-08-25 | |||||
| タイトル | ||||||
| タイトル | Radio-sensitivity of the LEC rat | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Ogiu, Toshiaki
× Ogiu, Toshiaki× Nishimura, Mayumi× Ishida, Yuka× Tsuji, Atsushi× Sugyou, Aya× Harada, Yoshinobu× Imai, Takashi× Hino, Okio× 荻生 俊昭× 西村 まゆみ× 石田 有香× 辻 厚至× 須尭 綾× 原田 良信× 今井 高志× 樋野 興夫 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The LEC rat is known to be a mutant strain which spontaneously develops heritable hepatitis due to copper accumulation, caused by mutation of the copper transporting ATPase gene (Atp7b). In addition, immunodefi ciency and radio-sensitivity have also been observed. Hayashi et al. extensively examined the radio-sensitivity of the LEC rat and concluded that its hypersensitivity is controlled by a single autosomal gene. Furthermore, they suggested the possibility that it correlates to copper accumulation due to the Atp7b gene mutation, because ionizing radiation-induced hydroxyl radicals might act in concert with copper-induced hydroxyl radicals. Firstly, we analyzed linkage between radio-sensitivity and the mutation responsible for LEC hepatitis in F1 animals of cross with the F344 rat. Our results clearly demonstrated an absence of any signifi cant association. In addition, partial dominance for radio-sensitivity was observed and radio-sensitive (F1 x LEC) backcross rats were twice as numerous as their radio-resistant counterparts, suggesting the possibility of control by two or more recessive genes. In order to select the radio-sensitivity gene in the LEC rat, we wished to develop congenic line of F344 rats using a phenotype-driven breeding protocol: rats of each backcross generation were mated with LEC rats and their progeny were irradiated with 4.5 Gy X rays, which dose cause death in LEC rats but did not in F344 rats, and judged 30 days later. Lately we also combined using genomic-wide genotyping in each backcross generation and their progeny to select heterogeneous rat. Although congenic rat line is not yet established until March of 2003, backcross exceeded 16 generations. This radio-sensitivity gene was demonstrated to locate in the region within 10Mb on the rat chromosome 4. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 12th International Congress of Radiation Research | |||||
| 発表年月日 | ||||||
| 日付 | 2003-08-22 | |||||
| 日付タイプ | Issued | |||||
