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ヒトがん及び正常細胞間におけるバイスタンダー経路に関する定量的解析
https://repo.qst.go.jp/records/55804
https://repo.qst.go.jp/records/55804511c40ed-e7c9-4702-979c-d67714644fd0
| Item type | 一般雑誌記事 / Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2012-12-14 | |||||
| タイトル | ||||||
| タイトル | ヒトがん及び正常細胞間におけるバイスタンダー経路に関する定量的解析 | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
小林, 亜利紗
× 小林, 亜利紗× 塩見, 尚子× 前田, 武× 児玉, 久美子× 鬼頭, 靖司× パンディー, バッドリ× 小西, 輝昭× 小林 亜利紗× 塩見 尚子× 前田 武× 児玉 久美子× 鬼頭 靖司× パンディー バッドリ× 小西 輝昭 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Radiation-induced bystander-effects (BEs), which are observed in non-irradiated cells that neighbor the irradiated cells, are known to be significant in living organisms. Radiation-induced BEs are generally classified into two major pathways: the gap-junction intercellular communication (GJIC) pathway and the cell-to-cell non-contact media transfer (MT) pathway. Generally, these two pathways are not investigated independently, but rather are investigated together. This may lead to misunderstandings regarding the mechanism of BEs, particularly in two different cell lines, such as cancer cells and normal cells. In this study, we examined the BEs among three different cell types: the human lung carcinoma cell lines A549 and A549-H2B-GFP, and the normal human lung fibroblast cell line WI-38. We found that, compared to co-culturing with A549 cells, co-culturing with WI38 cells enhanced the repair velocity of A549-H2B-GFP cells targeted with micro-beam irradiation. In addition, the survival of A549-H2B-GFP cells, which were co-cultured with WI-38, was increased by BEs signaling of the MT pathway. However, contrary to these results, measurement of the survival fraction, including the GJIC, showed that A549-H2B-GFP cells that were densely co-cultured with WI-38 cells had a lower survival fraction compared to A549-H2B-GFP cells co-cultured with A549 cells. These results indicate that there may be a "rescue" effect in bystander signals emitted through the MT pathway from the non-irradiated cells. |
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| 書誌情報 |
放射線医学総合研究所技術報告書 巻 2012, 号 NIRS-M-253, p. 17-25, 発行日 2012-11 |
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