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DNA conformational transitions inferred from reevaluation of m|Fo|-D|Fc| electron density maps
https://repo.qst.go.jp/records/48621
https://repo.qst.go.jp/records/486216ad8e59d-2967-4b4e-bc34-4d6d5dafb934
Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2018-03-23 | |||||
タイトル | ||||||
タイトル | DNA conformational transitions inferred from reevaluation of m|Fo|-D|Fc| electron density maps | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
著者 |
Sunami, Tomoko
× Sunami, Tomoko× Chatake, Toshiyuki× Kono, Hidetoshi× 角南 智子× 河野 秀俊 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Conformational flexibility of DNA plays important roles in biological processes such as transcriptional regulation and DNA packaging etc. To understand the mechanisms of these processes, it is important to analyse when, where and how DNA shows conformational variations. Recent analyses have indicated that conventional refinement methods do not always provide accurate models of crystallographic heterogeneities and that some information on polymorphism has been overlooked in previous crystallographic studies. In the present study, the m|Fo| D|Fc| electron-density maps of double-helical DNA crystal structures were calculated at a resolution equal to or better than 1.5 A ˚ and potential conformational transitions were found in 27% of DNA phosphates. Detailed analyses of the m|Fo| D|Fc| peaks indicated that some of these unassigned densities correspond to ZI $ ZII or A/B ! BI conformational transitions. A relationship was also found between ZI/ZII transitions and metal coordination in Z-DNA from the detected peaks. The present study highlights that frequent transitions of phosphate backbones occur even in crystals and that some of these transitions are affected by the local molecular environment. | |||||
書誌情報 |
Acta Crystallographica Section D 巻 73, 号 7, p. 600-608, 発行日 2017-07 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 2059-7983 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1107/S2059798317007707 |