WEKO3
アイテム
細胞核内DNA二本鎖切断部位の蛍光標識によるイオントラックの可視化
https://repo.qst.go.jp/records/48447
https://repo.qst.go.jp/records/48447bc511f6a-9d30-45e0-8a20-99c8f3cf5144
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2018-02-04 | |||||
| タイトル | ||||||
| タイトル | 細胞核内DNA二本鎖切断部位の蛍光標識によるイオントラックの可視化 | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
小西, 輝昭
× 小西, 輝昭× 竹安, 明浩× 雨宮, 邦招× 夏目, 敏之× 安田, 仲宏× 檜枝, 光太郎× 小西 輝昭× 竹安 明弘× 安田 仲宏× 檜枝 光太郎 |
|||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The geometric locations of ion traversals in mammalian cells constitute important information in the study of heavy ion-induced biological effects. We will introduce two methods for detecting heavy ion tracks in a nucleus of mammalian cells. The cells were irradiated with Ar ions to visualize the ions traversals by detecting the 3'-OH termini of DNA, where DSBs were induced, which were labeled with BrdU-triphosphate catalyzed by terminal deoxynucleotidyl transferase (TdT). This method of TUNEL is based on the specific binding of TdT to 3'-OH termini of DNA. Then, immuno-fluoresecent staining was performed against BrdU, and the images of the cells nuclei were acquired by confocal laser microscope. Another method is the contact microscopy technique, which was previously reported by Amemiya et al (2005), which enables to visualize simultaneously the images of cells as a relief on a plastic track detector, CR-39, and the etch pits which indicate the positions of ion traversals. We applied this method with immuno-staining assay against gamma-H2AX, which were used as a DNA double strand breaks (DSBs) marker, to compare geometric position of DSBs with the position of the ion traversals in the cell nuclei. | |||||
| 書誌情報 |
放射線 巻 33, 号 3, p. 201-210, 発行日 2007-07 |
|||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0285-3604 | |||||
