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Controlled in-cell release of caspase from photodegradable nanoparticles using the PARCEL method
https://repo.qst.go.jp/records/48210
https://repo.qst.go.jp/records/48210fc056e11-eb49-4fc4-b2e6-f10ca5041f37
Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2017-07-26 | |||||
タイトル | ||||||
タイトル | Controlled in-cell release of caspase from photodegradable nanoparticles using the PARCEL method | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
著者 |
Shibata, Yuka
× Shibata, Yuka× Murayama, Shuhei× Amamoto, Takaki× Santa, Tomofumi× Kato, Masaru× 村山 周平 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Although local protein activity in the cells is one of the hot topics in life science study, it still has many difficulties to measure or change its activity. Here, we prepared 20 nm-sized protein (caspase)-encapsulated photodegradable nanoparticles and used them to control the amount of caspase released within HeLa cells. Encapsulated caspase was released into the cytosol following irradiation. The increased amount of released caspase led to enhanced cell membrane permeability. These results indicate that the amount of protein released within cells can be regulated by the amount of protein-encapsulated nanoparticles; therefore, this technique would be useful for detailed study of protein function within cells or the control of cellular functions. |
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書誌情報 |
Appl. Spectrosc. Rev. 巻 51, 号 7-9, p. 669-677, 発行日 2016-03 |
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出版者 | ||||||
出版者 | Taylor & Francis Group | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1080/05704928.2016.1167068 |