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Reproducibility of measuring cerebral blood flow by laser-Doppler flowmetry in mice
https://repo.qst.go.jp/records/46923
https://repo.qst.go.jp/records/46923fa85bd86-a5f0-443a-92fd-1b2157fdc3cf
Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2014-11-10 | |||||
タイトル | ||||||
タイトル | Reproducibility of measuring cerebral blood flow by laser-Doppler flowmetry in mice | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
著者 |
Tajima, Yosuke
× Tajima, Yosuke× Takuwa, Hiroyuki× Kawaguchi, Hiroshi× Masamoto, Kazuto× Ikoma, Yoko× Seki, Chie× Taniguchi, Junko× Kanno, Iwao× Saeki, Naokatsu× Ito, Hiroshi× 田島 洋佑× 田桑 弘之× 川口 拓之× 正本 和人× 生駒 洋子× 関 千江× 谷口 順子× 菅野 巖× 伊藤 浩 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Laser-Doppler flowmetry has been widely used to trace hemodynamic changes in experimental stroke research. The purpose of the present study was to evaluate the day-to-day test-retest reproducibility of measuring cerebral blood flow by LDF in awake mice. The flux indicating cerebral blood flow (CBF), red blood cell (RBC) velocity, and RBC concentration were measured with LDF via cranial windows for the bilateral somatosensory cortex in awake mice. LDF measurements were performed three times, at baseline, 1 hour after, and 7 days after the baseline measurement. Moreover, breathing rate (BR) and partial pressure of transcutaneous CO2 (PtCO2) were measured simultaneously with LDF measurement. Intraclass correlation coefficient (ICC) and within-subject coefficient of variation (CVw) were calculated. CBF, RBC velocity, and RBC concentration showed good day-to-day test-retest reproducibility (ICC: 0.61 - 0.95, CVw: 8.3% - 15.4%). BR and PtCO2 in awake mice were stable during the course of the experiments. The evaluation of cerebral microcirculation using LDF appears to be applicable to long-term studies. | |||||
書誌情報 |
Frontiers in Bioscience Elite Edition 巻 6E, 号 1, p. 62-68, 発行日 2013-11 |
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出版者 | ||||||
出版者 | Frontiers in Bioscience | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1945-0494 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.2741/E691 | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | http://www.bioscience.org/2014/v6e/E691/fulltext.htm | |||||
関連名称 | http://www.bioscience.org/2014/v6e/E691/fulltext.htm |