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Superovulatory response, oocyte spontaneous activation, and embryo development in WMN/Nrs inbred rats
https://repo.qst.go.jp/records/45765
https://repo.qst.go.jp/records/457652058c639-82e1-4dd3-94e3-5cddc496b7c2
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2010-03-09 | |||||
| タイトル | ||||||
| タイトル | Superovulatory response, oocyte spontaneous activation, and embryo development in WMN/Nrs inbred rats | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Kito, Seiji
× Kito, Seiji× Yano, Hiroko× Oota, Yuki× Tsukamoto, Satoshi× 鬼頭 靖司× 矢野 浩子× 太田 有紀× 塚本 智史 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | WMN/Nrs inbred rats have been intensively used in radiation biology for years. However, their reproductive profile has never been examined. Here, we were examined various reproductive characteristics of WMN/Nrs inbred rats such as superovulatory response, oocytes spontaneous activation (OSA) and embryo development in vitro and in vivo. Superovulation was induced 3-9 weeks old females by injection of 150 IU/kg PMSG and 150 IU/Kg hCG by 48 h apart. Only 8 and 9 weeks old animals, but not younger age, superovulated average of 31.4 and 43.9 oocytes, respectively, and superovulation did not depend on the estrus cycle. Because Wistar strains are known to show high incidence of the OSA, factors expected to affect the OSA in WMN/Nrs, including time interval of various steps from euthanasia to oocyte recovery, incubation media, estrus cycle, anesthetic treatments and estrus cycle, were examined. Results showed time of animal euthanasia to oviduct excision was only factors affecting the OSA. Then, in vitro and in vivo embryo developmental competence was compared between embryos obtained by natural ovulation and superovulation. Although in vitro development of 2-cell embryos to blastocyst were similar levels between embryos obtained by natural ovulation (63.7%) and superovulation (69.7%), fetus development after embryo transfer was significantly lower in embryos obtained by superovulation (60.2%) than those by natural ovulation (87.5%, P=0.02). Our results provided important normative data for further application of rat assisted reproductive technologies (ARTs) such as in vitro fertilization and cryopreservation applicable to other strains as well as WMN/Nrs strain. | |||||
| 書誌情報 |
Experimental Animals 巻 59, 号 1, p. 35-45, 発行日 2010-03 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1341-1357 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | http://www.jstage.jst.go.jp/article/expanim/58/1/58_1/_article/-char/ja/ | |||||
| 関連名称 | http://www.jstage.jst.go.jp/article/expanim/58/1/58_1/_article/-char/ja/ | |||||
