WEKO3
アイテム
{"_buckets": {"deposit": "e8a69071-c51b-45c0-bb8d-202c0f0013fd"}, "_deposit": {"created_by": 1, "id": "45214", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "45214"}, "status": "published"}, "_oai": {"id": "oai:repo.qst.go.jp:00045214", "sets": ["1"]}, "author_link": ["1011653", "1011649", "1011652", "1011650", "1011651"], "item_8_biblio_info_7": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2007-05", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "5", "bibliographicPageEnd": "571", "bibliographicPageStart": "563", "bibliographicVolumeNumber": "167", "bibliographic_titles": [{"bibliographic_title": "Radiation Research"}]}]}, "item_8_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "To clarify the relationship between CDC2 kinase activity and radiation-induced apoptosis, we examined whether the cyclin-dependent kinase (CDK) inhibitor purvalanol A enhanced radiation-induced apoptosis in gastric tumor cells. MKN45 cells exposed to 20 Gy of X rays increased the CDC2 kinase activity and the expression of regulatory proteins (phospho-CDC2 and cyclin B1) of the G2/M phase, followed by activation of the G2/M checkpoint, whereas the treatment of X-irradiated MKN45 cells with 20 microM purvalanol A suppressed the increase in the CDC2 kinase activity and expression of the G2/M-phase regulatory proteins and reduced the fraction of the cells in the G2/M phase in the cell cycle. Furthermore, this treatment resulted in not only a significant increase in radiation-induced apoptosis but also the loss of clonogenicity in both MKN45 (p53-wild) and MKN28 (p53-mutated) cells. The expression of anti-apoptosis proteins, inhibitor of apoptosis protein (IAP) family members (survivin and XIAP) and BCL2 family members (Bcl-X(L) and Bcl-2), in purvalanol A-treated cells with and without X rays was significantly lower than for cells exposed to X rays alone. These results suggest that the inhibition of radiation-induced CDC2 kinase activity by purvalanol A induces apoptosis through the enhancement of active fragments of caspase 3.", "subitem_description_type": "Abstract"}]}, "item_8_relation_13": {"attribute_name": "PubMed番号", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "17474786", "subitem_relation_type_select": "PMID"}}]}, "item_8_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "10.1667/rr0622.1", "subitem_relation_type_select": "DOI"}}]}, "item_8_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0033-7587", "subitem_source_identifier_type": "ISSN"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "metadata only access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_14cb"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Iizuka, Daisuke"}], "nameIdentifiers": [{"nameIdentifier": "1011649", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Inanami, Osamu"}], "nameIdentifiers": [{"nameIdentifier": "1011650", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kashiwakura, Ikuo"}], "nameIdentifiers": [{"nameIdentifier": "1011651", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kuwabara, Mikinori"}], "nameIdentifiers": [{"nameIdentifier": "1011652", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Daisuke, Iizuka", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "1011653", "nameIdentifierScheme": "WEKO"}]}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Purvalanol A enhances cell killing by inhibiting up-regulation of Cdc2 kinase activity in tumor cells irradiated with high doses of X rays", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Purvalanol A enhances cell killing by inhibiting up-regulation of Cdc2 kinase activity in tumor cells irradiated with high doses of X rays"}]}, "item_type_id": "8", "owner": "1", "path": ["1"], "permalink_uri": "https://repo.qst.go.jp/records/45214", "pubdate": {"attribute_name": "公開日", "attribute_value": "2007-12-04"}, "publish_date": "2007-12-04", "publish_status": "0", "recid": "45214", "relation": {}, "relation_version_is_last": true, "title": ["Purvalanol A enhances cell killing by inhibiting up-regulation of Cdc2 kinase activity in tumor cells irradiated with high doses of X rays"], "weko_shared_id": -1}
Purvalanol A enhances cell killing by inhibiting up-regulation of Cdc2 kinase activity in tumor cells irradiated with high doses of X rays
https://repo.qst.go.jp/records/45214
https://repo.qst.go.jp/records/452146e37a19b-cb88-4f37-8392-87f5e1776a5b
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2007-12-04 | |||||
タイトル | ||||||
タイトル | Purvalanol A enhances cell killing by inhibiting up-regulation of Cdc2 kinase activity in tumor cells irradiated with high doses of X rays | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
著者 |
Iizuka, Daisuke
× Iizuka, Daisuke× Inanami, Osamu× Kashiwakura, Ikuo× Kuwabara, Mikinori× Daisuke, Iizuka |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | To clarify the relationship between CDC2 kinase activity and radiation-induced apoptosis, we examined whether the cyclin-dependent kinase (CDK) inhibitor purvalanol A enhanced radiation-induced apoptosis in gastric tumor cells. MKN45 cells exposed to 20 Gy of X rays increased the CDC2 kinase activity and the expression of regulatory proteins (phospho-CDC2 and cyclin B1) of the G2/M phase, followed by activation of the G2/M checkpoint, whereas the treatment of X-irradiated MKN45 cells with 20 microM purvalanol A suppressed the increase in the CDC2 kinase activity and expression of the G2/M-phase regulatory proteins and reduced the fraction of the cells in the G2/M phase in the cell cycle. Furthermore, this treatment resulted in not only a significant increase in radiation-induced apoptosis but also the loss of clonogenicity in both MKN45 (p53-wild) and MKN28 (p53-mutated) cells. The expression of anti-apoptosis proteins, inhibitor of apoptosis protein (IAP) family members (survivin and XIAP) and BCL2 family members (Bcl-X(L) and Bcl-2), in purvalanol A-treated cells with and without X rays was significantly lower than for cells exposed to X rays alone. These results suggest that the inhibition of radiation-induced CDC2 kinase activity by purvalanol A induces apoptosis through the enhancement of active fragments of caspase 3. | |||||
書誌情報 |
Radiation Research 巻 167, 号 5, p. 563-571, 発行日 2007-05 |
|||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0033-7587 | |||||
PubMed番号 | ||||||
識別子タイプ | PMID | |||||
関連識別子 | 17474786 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1667/rr0622.1 |