WEKO3
アイテム
{"_buckets": {"deposit": "311e1794-c189-4333-8a01-d38529c7ca94"}, "_deposit": {"created_by": 1, "id": "43561", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "43561"}, "status": "published"}, "_oai": {"id": "oai:repo.qst.go.jp:00043561", "sets": ["1"]}, "author_link": ["433142", "433146", "433139", "433143", "433144", "433141", "433138", "433145", "433137", "433147", "433135", "433140", "433136"], "item_8_biblio_info_7": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2004", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "2", "bibliographicPageEnd": "597", "bibliographicPageStart": "591", "bibliographicVolumeNumber": "60", "bibliographic_titles": [{"bibliographic_title": "International Journal of Radiation Oncology Biology Physics"}]}]}, "item_8_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "PURPOSE: We analyzed the death pattern of human lung cancer cells harboring different p53 statuses after irradiation with different levels of linear energy transfer (LET). METHODS AND MATERIALS: We used three kinds of human lung cancer cell lines with identical genotypes, except for the p53 gene. These cells were exposed to X-rays or accelerated carbon-ion beams. The cellular sensitivities were determined by a colony-forming assay. The detection and quantification of cell death (apoptosis and necrosis) were evaluated and compared by acridine orange/ethidium bromide double staining for fluorescence microscopy. RESULTS: We found that (1) there was no significant difference in cellular sensitivity to LET radiation \u003e70 KeV/microm, although wild-type p53 cell sensitivity to X-rays was higher than that of mutated p53 or p53-null cells; (2) low-LET radiation effectively induced apoptosis in wild-type p53 cells as compared with mutated p53 and p53-null cells; and (3) high-LET radiation induced p53-independent apoptosis. CONCLUSIONS: Our findings suggest that high-LET radiotherapy is expected to be a valid application for patients carrying mutated p53 cancer cells. We proposed that the elucidation of the p53-independent apoptosis-related genes might provide new insights into radiotherapy for cancer.", "subitem_description_type": "Abstract"}]}, "item_8_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0360-3016", "subitem_source_identifier_type": "ISSN"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "metadata only access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_14cb"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Takahashi, Akihisa"}], "nameIdentifiers": [{"nameIdentifier": "433135", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Matsumoto, Hideki"}], "nameIdentifiers": [{"nameIdentifier": "433136", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Aoki, Mizuho"}], "nameIdentifiers": [{"nameIdentifier": "433137", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Furusawa, Yoshiya"}], "nameIdentifiers": [{"nameIdentifier": "433138", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ohnishi, Ken"}], "nameIdentifiers": [{"nameIdentifier": "433139", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ohnishi, Takeo"}], "nameIdentifiers": [{"nameIdentifier": "433140", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "et.al"}], "nameIdentifiers": [{"nameIdentifier": "433141", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "高橋 昭久", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "433142", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "松本 英樹", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "433143", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "青木 瑞穂", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "433144", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "古澤 佳也", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "433145", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "大西 健", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "433146", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "大西 武雄", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "433147", "nameIdentifierScheme": "WEKO"}]}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "High-LET radiation enhanced apoptosis but not necrosis regardless of p53 status", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "High-LET radiation enhanced apoptosis but not necrosis regardless of p53 status"}]}, "item_type_id": "8", "owner": "1", "path": ["1"], "permalink_uri": "https://repo.qst.go.jp/records/43561", "pubdate": {"attribute_name": "公開日", "attribute_value": "2004-12-11"}, "publish_date": "2004-12-11", "publish_status": "0", "recid": "43561", "relation": {}, "relation_version_is_last": true, "title": ["High-LET radiation enhanced apoptosis but not necrosis regardless of p53 status"], "weko_shared_id": -1}
High-LET radiation enhanced apoptosis but not necrosis regardless of p53 status
https://repo.qst.go.jp/records/43561
https://repo.qst.go.jp/records/435612c22770f-9c61-4d38-a908-3668e1f07847
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2004-12-11 | |||||
タイトル | ||||||
タイトル | High-LET radiation enhanced apoptosis but not necrosis regardless of p53 status | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
著者 |
Takahashi, Akihisa
× Takahashi, Akihisa× Matsumoto, Hideki× Aoki, Mizuho× Furusawa, Yoshiya× Ohnishi, Ken× Ohnishi, Takeo× et.al× 高橋 昭久× 松本 英樹× 青木 瑞穂× 古澤 佳也× 大西 健× 大西 武雄 |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | PURPOSE: We analyzed the death pattern of human lung cancer cells harboring different p53 statuses after irradiation with different levels of linear energy transfer (LET). METHODS AND MATERIALS: We used three kinds of human lung cancer cell lines with identical genotypes, except for the p53 gene. These cells were exposed to X-rays or accelerated carbon-ion beams. The cellular sensitivities were determined by a colony-forming assay. The detection and quantification of cell death (apoptosis and necrosis) were evaluated and compared by acridine orange/ethidium bromide double staining for fluorescence microscopy. RESULTS: We found that (1) there was no significant difference in cellular sensitivity to LET radiation >70 KeV/microm, although wild-type p53 cell sensitivity to X-rays was higher than that of mutated p53 or p53-null cells; (2) low-LET radiation effectively induced apoptosis in wild-type p53 cells as compared with mutated p53 and p53-null cells; and (3) high-LET radiation induced p53-independent apoptosis. CONCLUSIONS: Our findings suggest that high-LET radiotherapy is expected to be a valid application for patients carrying mutated p53 cancer cells. We proposed that the elucidation of the p53-independent apoptosis-related genes might provide new insights into radiotherapy for cancer. | |||||
書誌情報 |
International Journal of Radiation Oncology Biology Physics 巻 60, 号 2, p. 591-597, 発行日 2004 |
|||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0360-3016 |