量研学術機関リポジトリ「QST-Repository」は、国立研究開発法人 量子科学技術研究開発機構に所属する職員等が生み出した学術成果（学会誌発表論文、学会発表、研究開発報告書、特許等）を集積しインターネット上で広く公開するサービスです。 Welcome to QST-Repository where we accumulates and discloses the academic research results(Journal Publications, Conference presentation, Research and Development Report, Patent, etc.) of the members of National Institutes for Quantum Science and Technology.
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Non-thermal plasma (NTP) devices have been explored for medical applications. NTP devices discharge electrons, positive ions, ultraviolet and reactive oxygen species (ROS) and reactive nitrogen species (RNS), such as hydroxyl radical (●OH), singlet oxygen (1O2), superoxide (O2●-), hydrogen peroxide (H2O2), ozone and nitric oxide at near physiological temperature. In preclinical stages or human clinical trials, NTP promotes blood coagulation, eradication of bacterial, viral and biofilm-related infections, wound healing and cancer cell death. Here, we detected ferric, vanadium and gold(III) ions significantly elevated lipid peroxidation, which were measured by 2-thiobarbituric acid-reactive substances (TBARS) in combination with NTP exposure. Using 3,3,5,5-tetramethyl-1-pyrroline-N-oxide (M4PO) as a spin probe, we identified tetrachloroaurate (III) yielded M4PO-X spin adduct with electron paramagnetic resonance (EPR). Tetrachloroaurate-induced oxidation was attenuated efficiently by reduced glutathione (GSH) and oxidized (GSSG), while glycine (Gly) and L-glutamate (Glu), which are constituent parts of GSH, were ineffective. Furthermore, GSH and GSSG efficiently suppressed tetrachloroaurate-induced lipid peroxidation, while Gly and Glu were also ineffective to suppress the elevation of TBARS. These results indicate that tetrachloroaurate-induced oxidation were attenuated by GSH as well as GSSG. Further studies were warranted to elucidate the redox reactions between metal ions and biomolecules that put forward the clinical application of NTP.
Tetrachloroaurate (III)–induced oxidation increases non-thermal plasma-induced oxidative stress
