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A549 and NCI-H460 cells, human non-small cell lung cancer (NSCLC) cell lines, were irradiated with X-ray or high linear energy transfer (LET) irradiation with or without ANTP-SMACN7 treatment. Cellular survival, apoptosis, and protein expression were studied by colony formation assays, flow cytometry, and western blot analyses, respectively.\nResults: ANTP-SMACN7 fusion proteins entered the cells and promoted A549 and NCI-H460 cell high LET irradiation radiosensitization. High LET irradiation was more efficient for clonogenic cell killing and the induction of apoptosis (P \u003c 0.05). Treatment with ANTP-SMACN7 significantly reduced the A549 and NCI-H460 cell clone-forming percentages and increased apoptosis through inhibition of the X-linked inhibitor of apoptosis protein and the activation of caspase-3 and caspase-9.\nConclusions: Regarding pharmaceutical radiosensitization, these findings provided a way to improve high-LET clinical radiotherapy for NSCLC patients.\nKeywords: Fe-particle radiation; carbon-particle radiation; caspase; non-small cell lung cancer cells; radiosensitizer.\n", "subitem_description_type": "Abstract"}]}, "item_8_relation_13": {"attribute_name": "PubMed番号", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "34546667", "subitem_relation_type_select": "PMID"}}]}, "item_8_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "10.20892/j.issn.2095-3941.2020.0569", "subitem_relation_type_select": "DOI"}}]}, "item_8_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "2095-3941", "subitem_source_identifier_type": "ISSN"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "metadata only access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_14cb"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Xie, Yi"}], "nameIdentifiers": [{"nameIdentifier": "1006740", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Wang, Bing"}], "nameIdentifiers": [{"nameIdentifier": "1006741", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Du, Liqing"}], "nameIdentifiers": [{"nameIdentifier": "1006742", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Wang, Yan"}], "nameIdentifiers": [{"nameIdentifier": "1006743", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Xu, Chang"}], "nameIdentifiers": [{"nameIdentifier": "1006744", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Zhang, Hong"}], "nameIdentifiers": [{"nameIdentifier": "1006745", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Wen, Kaixue"}], "nameIdentifiers": [{"nameIdentifier": "1006746", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Liu, Qiang"}], "nameIdentifiers": [{"nameIdentifier": "1006747", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Katsube, Takanori"}], "nameIdentifiers": [{"nameIdentifier": "1006748", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Bing, Wang", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "1006749", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hong, Zhang", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "1006750", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Takanori, Katsube", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "1006751", "nameIdentifierScheme": "WEKO"}]}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "ANTP-SMACN7 fusion peptide alone induced high linear energy transfer irradiation radiosensitization in non-small cell lung cancer cell lines.", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "ANTP-SMACN7 fusion peptide alone induced high linear energy transfer irradiation radiosensitization in non-small cell lung cancer cell lines."}]}, "item_type_id": "8", "owner": "1", "path": ["1"], "permalink_uri": "https://repo.qst.go.jp/records/83551", "pubdate": {"attribute_name": "公開日", "attribute_value": "2021-10-05"}, "publish_date": "2021-10-05", "publish_status": "0", "recid": "83551", "relation": {}, "relation_version_is_last": true, "title": ["ANTP-SMACN7 fusion peptide alone induced high linear energy transfer irradiation radiosensitization in non-small cell lung cancer cell lines."], "weko_shared_id": -1}
ANTP-SMACN7 fusion peptide alone induced high linear energy transfer irradiation radiosensitization in non-small cell lung cancer cell lines.
https://repo.qst.go.jp/records/83551
https://repo.qst.go.jp/records/83551e271773a-33e8-4443-985c-f5a7ee5ee485
Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2021-10-05 | |||||
タイトル | ||||||
タイトル | ANTP-SMACN7 fusion peptide alone induced high linear energy transfer irradiation radiosensitization in non-small cell lung cancer cell lines. | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
著者 |
Xie, Yi
× Xie, Yi× Wang, Bing× Du, Liqing× Wang, Yan× Xu, Chang× Zhang, Hong× Wen, Kaixue× Liu, Qiang× Katsube, Takanori× Bing, Wang× Hong, Zhang× Takanori, Katsube |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Objective: The aim of the present study was to investigate the mechanisms responsible for the radiation-sensitizing effect of antennapedia proteins, ANTP-SMACN7, on lung cancer cells treated with accelerated carbon and Fe particle irradiation. Methods: The ANTP-SMACN7 fusion peptide was synthesized and linked to fluorescein isothiocyanate to determine its ability to penetrate cells. A549 and NCI-H460 cells, human non-small cell lung cancer (NSCLC) cell lines, were irradiated with X-ray or high linear energy transfer (LET) irradiation with or without ANTP-SMACN7 treatment. Cellular survival, apoptosis, and protein expression were studied by colony formation assays, flow cytometry, and western blot analyses, respectively. Results: ANTP-SMACN7 fusion proteins entered the cells and promoted A549 and NCI-H460 cell high LET irradiation radiosensitization. High LET irradiation was more efficient for clonogenic cell killing and the induction of apoptosis (P < 0.05). Treatment with ANTP-SMACN7 significantly reduced the A549 and NCI-H460 cell clone-forming percentages and increased apoptosis through inhibition of the X-linked inhibitor of apoptosis protein and the activation of caspase-3 and caspase-9. Conclusions: Regarding pharmaceutical radiosensitization, these findings provided a way to improve high-LET clinical radiotherapy for NSCLC patients. Keywords: Fe-particle radiation; carbon-particle radiation; caspase; non-small cell lung cancer cells; radiosensitizer. |
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書誌情報 |
Cancer biology & medicine 発行日 2021-09 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 2095-3941 | |||||
PubMed番号 | ||||||
識別子タイプ | PMID | |||||
関連識別子 | 34546667 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.20892/j.issn.2095-3941.2020.0569 |