量研学術機関リポジトリ「QST-Repository」は、国立研究開発法人 量子科学技術研究開発機構に所属する職員等が生み出した学術成果（学会誌発表論文、学会発表、研究開発報告書、特許等）を集積しインターネット上で広く公開するサービスです。 Welcome to QST-Repository where we accumulates and discloses the academic research results(Journal Publications, Conference presentation, Research and Development Report, Patent, etc.) of the members of National Institutes for Quantum and Radiological Science and Technology.
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Characterization of chlorophyll-deficient soybean [Glycine max (L.) Merr.] mutants obtained by ion-beam irradiation reveals concomitant reduction in isoflavone levels
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Ion-beam irradiation serves as a powerful tool of mutagenesis that engineers novel traits in a wide range of plants. A previous study indicated that coincidental changes of multiple traits are frequently induced by ion-beam irradiation in soybean, a paleopolyploid plant whose genome comprises a large number of duplicated genes. Here we analyzed the levels of isoflavones, the major secondary compounds accumulated in soybean seeds, in a population of mutants having chlorophyll deficiency of various degrees obtained by the mutagenesis. Significant reduction in the levels of four isoflavones, namely, daidzin, genistin, and their malonylglucosides, was detected in 4–9 plant lines among 28 lines analyzed. The average levels of chlorophyll in the leaf tissues and isoflavones in seeds are correlated with each other. In selected mutant lines, the levels of isoflavones were lower than the wild-type plants throughout seed development. Gene expression profiles indicated that the mRNA levels of multiple genes involved in the isoflavone synthesis pathway reduced during seed development, which suggests that the observed reduction in the levels of isoflavones may be attributable to gene expression changes. Along with the possibility that multiple and independent mutations that reduce the levels of chlorophyll and isoflavones occurred simultaneously, these observations suggest the presence of an unrecognized physiological link between the synthesis and/or accumulation of these compounds in soybean.
Characterization of chlorophyll-deficient soybean [Glycine max (L.) Merr.] mutants obtained by ion-beam irradiation reveals concomitant reduction in isoflavone levels
