WEKO3
アイテム
{"_buckets": {"deposit": "a771c9f2-0b7b-4c9b-9ac7-d57e26534f3a"}, "_deposit": {"created_by": 1, "id": "80155", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "80155"}, "status": "published"}, "_oai": {"id": "oai:repo.qst.go.jp:00080155", "sets": ["1"]}, "author_link": ["888991", "888994", "888987", "888992", "888993", "888988", "888989", "888990"], "item_8_biblio_info_7": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2020-09", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "5", "bibliographicPageEnd": "2441", "bibliographicPageStart": "2437", "bibliographicVolumeNumber": "34", "bibliographic_titles": [{"bibliographic_title": "in vivo"}]}]}, "item_8_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Background: Ascorbate peroxidase (APX) derived from Cyanidioschyzon merolae, a primitive red alga living in high temperature and acidic environments, has a greater anti-oxidative capacity than similar peroxidases occurring in other plants. In the present study, we examined the ability of Cyanidioschyzon merolae-derived APX (cAPX) to increase anti-oxidative capacity when expressed in mammalian cells. Materials and Methods: The cAPX gene was introduced into the mouse fibroblast-like cell line C3H10T1/2. Production of reactive oxygen species (ROS) and/or cell viability was assessed after heat, H2O2 and acid stimulation. Results: Heat and H2O2 stimulation caused ROS production. cAPX-expressing cells were more tolerant to oxidative stress induced by heat, H2O2 and acid stimulations than control cells lacking cAPX. Conclusion: Introduction of cAPX increases anti-oxidative capacity in mammalian cells.", "subitem_description_type": "Abstract"}]}, "item_8_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "10.21873/invivo.12058", "subitem_relation_type_select": "DOI"}}]}, "item_8_relation_17": {"attribute_name": "関連サイト", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "http://iv.iiarjournals.org/content/34/5/2437.full", "subitem_relation_type_select": "URI"}}]}, "item_8_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0258-851X", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Hitomi, Suzuro"}], "nameIdentifiers": [{"nameIdentifier": "888987", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kokabu, Shoichiro"}], "nameIdentifiers": [{"nameIdentifier": "888988", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ken-ichiro, Matsumoto"}], "nameIdentifiers": [{"nameIdentifier": "888989", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Shoji, Yoshimi"}], "nameIdentifiers": [{"nameIdentifier": "888990", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ujihara, Izumi"}], "nameIdentifiers": [{"nameIdentifier": "888991", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ono, Kentaro"}], "nameIdentifiers": [{"nameIdentifier": "888992", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Matsumoto, Kenichiro", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "888993", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Shoji, Yoshimi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "888994", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2020-09-25"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "75808c811c7089c9a2ccd3f2660d6987.pdf", "filesize": [{"value": "1.1 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 1100000.0, "url": {"label": "Hitomi.S.2020.In.Vivo.34.2437-2441.AscorbatePeroxidase.pdf", "url": "https://repo.qst.go.jp/record/80155/files/75808c811c7089c9a2ccd3f2660d6987.pdf"}, "version_id": "a5598c03-edfb-476e-ba3a-1c1eb789311f"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Expression of Ascorbate Peroxidase Derived from Cyanidioschyzon merolae in Mammalian Cells", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Expression of Ascorbate Peroxidase Derived from Cyanidioschyzon merolae in Mammalian Cells"}]}, "item_type_id": "8", "owner": "1", "path": ["1"], "permalink_uri": "https://repo.qst.go.jp/records/80155", "pubdate": {"attribute_name": "公開日", "attribute_value": "2020-06-08"}, "publish_date": "2020-06-08", "publish_status": "0", "recid": "80155", "relation": {}, "relation_version_is_last": true, "title": ["Expression of Ascorbate Peroxidase Derived from Cyanidioschyzon merolae in Mammalian Cells"], "weko_shared_id": -1}
Expression of Ascorbate Peroxidase Derived from Cyanidioschyzon merolae in Mammalian Cells
https://repo.qst.go.jp/records/80155
https://repo.qst.go.jp/records/801550795419b-377f-40ff-b229-4338145a1de1
名前 / ファイル | ライセンス | アクション |
---|---|---|
Hitomi.S.2020.In.Vivo.34.2437-2441.AscorbatePeroxidase.pdf (1.1 MB)
|
|
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2020-06-08 | |||||
タイトル | ||||||
タイトル | Expression of Ascorbate Peroxidase Derived from Cyanidioschyzon merolae in Mammalian Cells | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Hitomi, Suzuro
× Hitomi, Suzuro× Kokabu, Shoichiro× Ken-ichiro, Matsumoto× Shoji, Yoshimi× Ujihara, Izumi× Ono, Kentaro× Matsumoto, Kenichiro× Shoji, Yoshimi |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Background: Ascorbate peroxidase (APX) derived from Cyanidioschyzon merolae, a primitive red alga living in high temperature and acidic environments, has a greater anti-oxidative capacity than similar peroxidases occurring in other plants. In the present study, we examined the ability of Cyanidioschyzon merolae-derived APX (cAPX) to increase anti-oxidative capacity when expressed in mammalian cells. Materials and Methods: The cAPX gene was introduced into the mouse fibroblast-like cell line C3H10T1/2. Production of reactive oxygen species (ROS) and/or cell viability was assessed after heat, H2O2 and acid stimulation. Results: Heat and H2O2 stimulation caused ROS production. cAPX-expressing cells were more tolerant to oxidative stress induced by heat, H2O2 and acid stimulations than control cells lacking cAPX. Conclusion: Introduction of cAPX increases anti-oxidative capacity in mammalian cells. | |||||
書誌情報 |
in vivo 巻 34, 号 5, p. 2437-2441, 発行日 2020-09 |
|||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0258-851X | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.21873/invivo.12058 | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | http://iv.iiarjournals.org/content/34/5/2437.full |