WEKO3
アイテム
{"_buckets": {"deposit": "a3734f90-f3fc-41dc-88c1-fc0ecdcaeda1"}, "_deposit": {"created_by": 1, "id": "79303", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "79303"}, "status": "published"}, "_oai": {"id": "oai:repo.qst.go.jp:00079303", "sets": ["7"]}, "author_link": ["850129", "850130", "850133", "850134", "850135", "850128", "850131", "850132"], "item_10004_biblio_info_7": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2020-03", "bibliographicIssueDateType": "Issued"}, "bibliographicPageStart": "82", "bibliographicVolumeNumber": "QST-M-23", "bibliographic_titles": [{"bibliographic_title": "QST Takasaki Annual Report 2018"}]}]}, "item_10004_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Microalgae are promising biofuel producers due to their high ability to produce oil photosynthetically. The oleaginous green alga Chlamydomonas sp. JSC4 is a hopeful candidate that shows high growth potential together with high oil content through activation of the starch-to-lipid biosynthesis switching mechanism. However, oil accumulation of JSC4 is significantly decreased under light/dark conditions, which is inevitable in large-scale outdoor cultivation. To overcome this critical obstacle, this study aimed to obtain a mutant strain with high oil content under light/dark conditions. Selective breeding of oil-rich Chlamydomonas mutant was performed by combining heavy ion beam mutagenesis and fluorescence-activated cell sorting (FACS). JSC4 cells seeded on agar plates were exposed to 50 Gy of the carbon ion beams (12C5+, 220 MeV) accelerated by an AVF cyclotron at TIARA, QST. Mutant cells were cultured under light/dark conditions, followed by FACS-based screening for oil-rich cells using BODIPY fluorescence and chlorophyll fluorescence as the indicators of oil and cell size, respectively. Consequently, an oil-rich mutant strain KOR1 was obtained.", "subitem_description_type": "Abstract"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "metadata only access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_14cb"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Kato, Yuichi"}], "nameIdentifiers": [{"nameIdentifier": "850128", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ogino, Chiaki"}], "nameIdentifiers": [{"nameIdentifier": "850129", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hasunuma, Tomohisa"}], "nameIdentifiers": [{"nameIdentifier": "850130", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sato, Katsuya"}], "nameIdentifiers": [{"nameIdentifier": "850131", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ono, Yutaka"}], "nameIdentifiers": [{"nameIdentifier": "850132", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kondo, Akihiko"}], "nameIdentifiers": [{"nameIdentifier": "850133", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sato, Katsuya", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "850134", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ono, Yutaka", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "850135", "nameIdentifierScheme": "WEKO"}]}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Overcoming Decreased Lipid Accumulation Under Light/Dark Conditions by Selective Breeding of Oil-Rich Chlamydomonas Mutants", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Overcoming Decreased Lipid Accumulation Under Light/Dark Conditions by Selective Breeding of Oil-Rich Chlamydomonas Mutants"}]}, "item_type_id": "10004", "owner": "1", "path": ["7"], "permalink_uri": "https://repo.qst.go.jp/records/79303", "pubdate": {"attribute_name": "公開日", "attribute_value": "2020-03-11"}, "publish_date": "2020-03-11", "publish_status": "0", "recid": "79303", "relation": {}, "relation_version_is_last": true, "title": ["Overcoming Decreased Lipid Accumulation Under Light/Dark Conditions by Selective Breeding of Oil-Rich Chlamydomonas Mutants"], "weko_shared_id": -1}
Overcoming Decreased Lipid Accumulation Under Light/Dark Conditions by Selective Breeding of Oil-Rich Chlamydomonas Mutants
https://repo.qst.go.jp/records/79303
https://repo.qst.go.jp/records/79303095a831d-2090-4066-b689-a4daaef03d45
Item type | 一般雑誌記事 / Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2020-03-11 | |||||
タイトル | ||||||
タイトル | Overcoming Decreased Lipid Accumulation Under Light/Dark Conditions by Selective Breeding of Oil-Rich Chlamydomonas Mutants | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | article | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
著者 |
Kato, Yuichi
× Kato, Yuichi× Ogino, Chiaki× Hasunuma, Tomohisa× Sato, Katsuya× Ono, Yutaka× Kondo, Akihiko× Sato, Katsuya× Ono, Yutaka |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Microalgae are promising biofuel producers due to their high ability to produce oil photosynthetically. The oleaginous green alga Chlamydomonas sp. JSC4 is a hopeful candidate that shows high growth potential together with high oil content through activation of the starch-to-lipid biosynthesis switching mechanism. However, oil accumulation of JSC4 is significantly decreased under light/dark conditions, which is inevitable in large-scale outdoor cultivation. To overcome this critical obstacle, this study aimed to obtain a mutant strain with high oil content under light/dark conditions. Selective breeding of oil-rich Chlamydomonas mutant was performed by combining heavy ion beam mutagenesis and fluorescence-activated cell sorting (FACS). JSC4 cells seeded on agar plates were exposed to 50 Gy of the carbon ion beams (12C5+, 220 MeV) accelerated by an AVF cyclotron at TIARA, QST. Mutant cells were cultured under light/dark conditions, followed by FACS-based screening for oil-rich cells using BODIPY fluorescence and chlorophyll fluorescence as the indicators of oil and cell size, respectively. Consequently, an oil-rich mutant strain KOR1 was obtained. | |||||
書誌情報 |
QST Takasaki Annual Report 2018 巻 QST-M-23, p. 82, 発行日 2020-03 |