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Direct Observation of the Protonation States in the Mutant Green Fluorescent Protein
https://repo.qst.go.jp/records/78473
https://repo.qst.go.jp/records/784732872f753-4d18-482f-a61a-bd7afc155e79
Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2019-12-17 | |||||
タイトル | ||||||
タイトル | Direct Observation of the Protonation States in the Mutant Green Fluorescent Protein | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
著者 |
Shibazaki, Chie
× Shibazaki, Chie× Shimizu, Rumi× Kagotani, Yuji× Ostermann, Andreas× E. Schrader, Tobias× Adachi, Motoyasu× Chie, Shibazaki× Rumi, Shimizu× Yuji, Kagotani× Motoyasu, Adachi |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Neutron crystallography has been used to elucidate the protonation states for the enhanced green fluorescent protein, which has revolutionized the imaging technologies. The structure has a deprotonated hydroxyl group in the fluorescent chromophore. Also, the protonation states of His148 and Thr203, as well as the orientation of a critical water molecule in direct contact with the chromophore, could be determined. The results demonstrate that the deprotonated hydroxyl group in the chromophore and the nitrogen atom ND1 in His148 are charged negatively and positively, respectively, forming an ion pair. The position of the two deuterium atoms in the critical water molecule appears to be displaced slightly toward the acceptor oxygen atoms according to their omit maps. This displacement implies the formation of an intriguing electrostatic potential realized inside the protein. Our findings provide new insights into strategy for future protein design with developments for quantum chemical calculations. | |||||
書誌情報 |
The Journal of Physical Chemistry Letters 巻 11, 号 2, p. 492-496, 発行日 2020-01 |
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出版者 | ||||||
出版者 | ACS Publications | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1948-7185 | |||||
PubMed番号 | ||||||
識別子タイプ | PMID | |||||
関連識別子 | 31880458 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1021/acs.jpclett.9b03252 | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://pubs.acs.org/doi/10.1021/acs.jpclett.9b03252 |