量研学術機関リポジトリ「QST-Repository」は、国立研究開発法人 量子科学技術研究開発機構に所属する職員等が生み出した学術成果（学会誌発表論文、学会発表、研究開発報告書、特許等）を集積しインターネット上で広く公開するサービスです。 Welcome to QST-Repository where we accumulates and discloses the academic research results(Journal Publications, Conference presentation, Research and Development Report, Patent, etc.) of the members of National Institutes for Quantum Science and Technology.
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Biological markers, which indicate radiation effects and are used for predicting late effects in the future, are expected to be identified. The purpose of this study is to explore useful biological markers for evaluating radiation effects.
Particularly, low-dose particle radiation may induce bystander effects due to low particle fluence and to compare the changes in molecules after particle irradiation with that after X-rays irradiation might possibly detect particle-radiation specific biomarkers. Metabolic analyses were performed in livers of mice irradiated with X-rays. Dose-dependent or dose-specific alterations were evaluated. In addition to it, investigating differences of biomarkers due to radiation quality, specific alteration in metabolites was compared with that in the case of Fe-ion irradiation. Moreover, we tried to detect inflammatory biomarkers in both X-rays and Fe-ion irradiation.
Metabolites in livers of mice (C57BL/6J) were analyzed by capillary electrophoresis-time-of-flight mass spectrometry (CE-TOFMS) one month after X-ray irradiation. Significantly altered metabolites to the control were selected from the irradiation groups and specific trends were evaluated. One month after irradiation, 4, 8 and 16 metabolites were significantly altered in irradiated groups at doses of 0.1Gy, 0.5Gy and 2Gy, respectively, compared to the levels in the control. Significantly altered metabolites included free amino acids. However, changes in free amino acids after Fe-ion irradiation (0, 0.1 and 2 Gy) were not detected. These will be discussed in the viewpoint of amino acid metabolism and usage as biomarkers for evaluating radiation effects with results of cytokine alteration after irradiation.
Comparative exploration of biomarkers in mice exposed to X-ray or Fe-ion radiation
