量研学術機関リポジトリ「QST-Repository」は、国立研究開発法人 量子科学技術研究開発機構に所属する職員等が生み出した学術成果（学会誌発表論文、学会発表、研究開発報告書、特許等）を集積しインターネット上で広く公開するサービスです。 Welcome to QST-Repository where we accumulates and discloses the academic research results(Journal Publications, Conference presentation, Research and Development Report, Patent, etc.) of the members of National Institutes for Quantum Science and Technology.
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Radiation carcinogenic risk is greatly dependent on the age at exposure. To find characteristic molecular changes in tumors induced by radiation at young age, we used the mouse T-cell lymphoma (TL) model. TL was induced by fractionated whole-body X-irradiation (four weekly fractions) during infancy (starting at 1 week old), prepuberty (4 weeks old), and young adult (8 weeks old). We assessed the mutation of Ikaros and Pten in the TLs by the analyses of loss of heterozygosity (LOH), array-based comparative genome hybridization, sequencing and Western blotting. LOH at Ikaros loci and mutation of Ikaros were most frequent in the young adult irradiation group. In contrast, the frequency of LOH at Pten loci and mutation of Pten were the highest in the infant irradiation group. Thus, Ikaros mutations were implicated in T-cell lymphomagenesis in adult, while Pten mutations were predominant in infant. Moreover, the mechanisms of allele loss appeared different among these age groups. These results suggest that major tumor suppressor genes involved in radiation T-cell lymphomagenesis and the molecular mechanism of mutation change as a function of age at exposure.
Age dependent role of Ikaros and Pten mutations in radiation T-cell lymphomagenesis in mice
