WEKO3
アイテム
{"_buckets": {"deposit": "98964b49-069b-4b9c-9023-193571a31f84"}, "_deposit": {"created_by": 1, "id": "70162", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "70162"}, "status": "published"}, "_oai": {"id": "oai:repo.qst.go.jp:00070162", "sets": ["28"]}, "author_link": ["688946", "688947", "688940", "688945", "688948", "688950", "688951", "688949", "688952", "688944", "688941", "688943", "688942", "688939"], "item_10005_date_7": {"attribute_name": "発表年月日", "attribute_value_mlt": [{"subitem_date_issued_datetime": "2010-05-22", "subitem_date_issued_type": "Issued"}]}, "item_10005_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Purpose: Up to 2009, over 5000 patients have been treated in HIMAC. Malignant melanoma showed high local control of about 75%, whereas the overall survival was about 36% at 5-years. It is an important subject to control tumor distant metastasis for heavy-ion radiotherapy. The aim of this study is to clarify the effect of carbon ion beams (C-ion) on metastatic potential of melanoma in vitro and in vivo. \nMaterials and Methods: A highly metastatic mouse malignant melanoma cell line B16/BL6 were maintained in RPMI-1640 medium supplemented with 10% FBS and antibiotics. [in vitro] Samples were prepared 2 days before and then irradiated with C-ions at 13, 50 or 75 keV/um or X-rays. Surviving fractions were obtained using colony formation assay. Migration and invasion activity as metastatic potentials of the cells were examined using the Boyden-chamber method and the Matrigel invasion assay. [in vivo] The cells were implanted in right-leg of C57BL/6J mice at 1x106 cells/mouse 9-10 days before irradiation. Tumors were irradiated at the center of 6cm-SOBP of C-ions, or gamma-rays. Radiosensitivity for whole tumor was obtained by the tumor growth delay method, and that for individual cell in a tumor was obtained by an in vivo-in vitro assay. The metastatic effects were analyzed with the spontaneous lung metastasis model. \nResults: [in vitro] Survival curves showed higher cytotoxic effects of C-ions compared with X-rays, and the RBE values were 1.1, 1.7 or 2.5 at 13, 50, or 75 keV/um, respectively. The potential of migration and invasion were suppressed by C-ions at all dose points (0.25 to 8.0 Gy) tested, however it was enhanced by X-rays at low dose points (0.25 to 0.5Gy) than non-irradiated controls. The RBE values obtained from migration and invasion test were higher than that from cell killing. [in vivo] C-ions significantly suppressed the tumor growth, and the RBE was 2.6. The numbers of lung metastatic nodules after tumor-irradiations decreased with the dose, and C-ions were more effective compared with gamma-rays. The metastatic potentials of survived cells in a tumor after irradiation was analyzed with the number of metastatic lung colony from implanted tumors and survival of irradiated and explanted cells from a tumor. \nSmaller number of metastasis was found for C-ions than gamma-rays when the tumor cell survivals were 10%. \nConclusion: It might suggest that C-ion inhibit metastasis at radiotherapy compared with low-LET photons.", "subitem_description_type": "Abstract"}]}, "item_10005_description_6": {"attribute_name": "会議概要(会議名, 開催地, 会期, 主催者等)", "attribute_value_mlt": [{"subitem_description": "PTCOG 49", "subitem_description_type": "Other"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "metadata only access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_14cb"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Matsumoto, Yoshitaka"}], "nameIdentifiers": [{"nameIdentifier": "688939", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Koike, Sachiko"}], "nameIdentifiers": [{"nameIdentifier": "688940", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Uzawa, Akiko"}], "nameIdentifiers": [{"nameIdentifier": "688941", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hirayama, Ryoichi"}], "nameIdentifiers": [{"nameIdentifier": "688942", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Okayasu, Ryuichi"}], "nameIdentifiers": [{"nameIdentifier": "688943", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ando, Koichi"}], "nameIdentifiers": [{"nameIdentifier": "688944", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Furusawa, Yoshiya"}], "nameIdentifiers": [{"nameIdentifier": "688945", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "et.al"}], "nameIdentifiers": [{"nameIdentifier": "688946", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "松本 孔貴", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "688947", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "小池 幸子", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "688948", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "鵜澤 玲子", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "688949", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "平山 亮一", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "688950", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "岡安 隆一", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "688951", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "古澤 佳也", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "688952", "nameIdentifierScheme": "WEKO"}]}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "conference object", "resourceuri": "http://purl.org/coar/resource_type/c_c94f"}]}, "item_title": "The effects of heavy-ion and photon beams to mouse malignant melanoma cell line having highly metastatic potential", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "The effects of heavy-ion and photon beams to mouse malignant melanoma cell line having highly metastatic potential"}]}, "item_type_id": "10005", "owner": "1", "path": ["28"], "permalink_uri": "https://repo.qst.go.jp/records/70162", "pubdate": {"attribute_name": "公開日", "attribute_value": "2010-06-22"}, "publish_date": "2010-06-22", "publish_status": "0", "recid": "70162", "relation": {}, "relation_version_is_last": true, "title": ["The effects of heavy-ion and photon beams to mouse malignant melanoma cell line having highly metastatic potential"], "weko_shared_id": -1}
The effects of heavy-ion and photon beams to mouse malignant melanoma cell line having highly metastatic potential
https://repo.qst.go.jp/records/70162
https://repo.qst.go.jp/records/7016290211163-76cc-4b6d-b275-46fd4e398e1d
Item type | 会議発表用資料 / Presentation(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2010-06-22 | |||||
タイトル | ||||||
タイトル | The effects of heavy-ion and photon beams to mouse malignant melanoma cell line having highly metastatic potential | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
資源タイプ | conference object | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
著者 |
Matsumoto, Yoshitaka
× Matsumoto, Yoshitaka× Koike, Sachiko× Uzawa, Akiko× Hirayama, Ryoichi× Okayasu, Ryuichi× Ando, Koichi× Furusawa, Yoshiya× et.al× 松本 孔貴× 小池 幸子× 鵜澤 玲子× 平山 亮一× 岡安 隆一× 古澤 佳也 |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Purpose: Up to 2009, over 5000 patients have been treated in HIMAC. Malignant melanoma showed high local control of about 75%, whereas the overall survival was about 36% at 5-years. It is an important subject to control tumor distant metastasis for heavy-ion radiotherapy. The aim of this study is to clarify the effect of carbon ion beams (C-ion) on metastatic potential of melanoma in vitro and in vivo. Materials and Methods: A highly metastatic mouse malignant melanoma cell line B16/BL6 were maintained in RPMI-1640 medium supplemented with 10% FBS and antibiotics. [in vitro] Samples were prepared 2 days before and then irradiated with C-ions at 13, 50 or 75 keV/um or X-rays. Surviving fractions were obtained using colony formation assay. Migration and invasion activity as metastatic potentials of the cells were examined using the Boyden-chamber method and the Matrigel invasion assay. [in vivo] The cells were implanted in right-leg of C57BL/6J mice at 1x106 cells/mouse 9-10 days before irradiation. Tumors were irradiated at the center of 6cm-SOBP of C-ions, or gamma-rays. Radiosensitivity for whole tumor was obtained by the tumor growth delay method, and that for individual cell in a tumor was obtained by an in vivo-in vitro assay. The metastatic effects were analyzed with the spontaneous lung metastasis model. Results: [in vitro] Survival curves showed higher cytotoxic effects of C-ions compared with X-rays, and the RBE values were 1.1, 1.7 or 2.5 at 13, 50, or 75 keV/um, respectively. The potential of migration and invasion were suppressed by C-ions at all dose points (0.25 to 8.0 Gy) tested, however it was enhanced by X-rays at low dose points (0.25 to 0.5Gy) than non-irradiated controls. The RBE values obtained from migration and invasion test were higher than that from cell killing. [in vivo] C-ions significantly suppressed the tumor growth, and the RBE was 2.6. The numbers of lung metastatic nodules after tumor-irradiations decreased with the dose, and C-ions were more effective compared with gamma-rays. The metastatic potentials of survived cells in a tumor after irradiation was analyzed with the number of metastatic lung colony from implanted tumors and survival of irradiated and explanted cells from a tumor. Smaller number of metastasis was found for C-ions than gamma-rays when the tumor cell survivals were 10%. Conclusion: It might suggest that C-ion inhibit metastasis at radiotherapy compared with low-LET photons. |
|||||
会議概要(会議名, 開催地, 会期, 主催者等) | ||||||
内容記述タイプ | Other | |||||
内容記述 | PTCOG 49 | |||||
発表年月日 | ||||||
日付 | 2010-05-22 | |||||
日付タイプ | Issued |