WEKO3
アイテム
{"_buckets": {"deposit": "052fe00c-e9e9-4211-aae5-6d92e0fb5b41"}, "_deposit": {"created_by": 1, "id": "69563", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "69563"}, "status": "published"}, "_oai": {"id": "oai:repo.qst.go.jp:00069563", "sets": ["28"]}, "author_link": ["682785", "682791", "682782", "682783", "682787", "682790", "682786", "682788", "682784", "682789"], "item_10005_date_7": {"attribute_name": "発表年月日", "attribute_value_mlt": [{"subitem_date_issued_datetime": "2008-10-30", "subitem_date_issued_type": "Issued"}]}, "item_10005_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "In vivo electroporation (EP) is one of the efficient methods for effective gene transfer. Application of in vivo EP-mediated gene transfer (EGT) in anticancer gene therapy is highly expected since it could be performed repeatedly, safely and easily with low cost. Meanwhile, effective transfer, long-term expression and non-invasive monitoring are critical for optimal gene therapy. Here we report the feasibility of in vivo optical and MRI of EP-mediated gene expression in tumor model. Initially, we observed the in vivo EGT level and its temporal change by means of optical imaging using red fluorescence protein (RFP) as a reporter gene. Next, we constructed a dual reporter plasmid carrying a gene encoding ferritin heavy chain (FHC), a MRI reporter, and RFP gene to visualize transgene by dual modality. In cellular T2-weighted MRI, cells transfected with FHC plasmid showed lower signal intensity compared to the control cells. Moreover, after in vivo EGT, the plasmid-injected region in the tumor showed low signal intensity on T2-weighted MRI. Thus, our strategy would be a platform technology to evaluate EP-mediated gene therapy without necessity to administer contrast agent or substrate.", "subitem_description_type": "Abstract"}]}, "item_10005_description_6": {"attribute_name": "会議概要(会議名, 開催地, 会期, 主催者等)", "attribute_value_mlt": [{"subitem_description": "第67回日本癌学会学術総会", "subitem_description_type": "Other"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "metadata only access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_14cb"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "U, Winn Aung"}], "nameIdentifiers": [{"nameIdentifier": "682782", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hasegawa, Sumitaka"}], "nameIdentifiers": [{"nameIdentifier": "682783", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Koshikawa, Michiko"}], "nameIdentifiers": [{"nameIdentifier": "682784", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Furukawa, Takako"}], "nameIdentifiers": [{"nameIdentifier": "682785", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Saga, Tsuneo"}], "nameIdentifiers": [{"nameIdentifier": "682786", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "U Winn Aung", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "682787", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "長谷川 純崇", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "682788", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "越川 道子", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "682789", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "古川 高子", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "682790", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "佐賀 恒夫", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "682791", "nameIdentifierScheme": "WEKO"}]}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "conference object", "resourceuri": "http://purl.org/coar/resource_type/c_c94f"}]}, "item_title": "Dual-modality in vivo imaging of electroporation-mediated transgene expression in experimental tumors", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Dual-modality in vivo imaging of electroporation-mediated transgene expression in experimental tumors"}]}, "item_type_id": "10005", "owner": "1", "path": ["28"], "permalink_uri": "https://repo.qst.go.jp/records/69563", "pubdate": {"attribute_name": "公開日", "attribute_value": "2008-10-31"}, "publish_date": "2008-10-31", "publish_status": "0", "recid": "69563", "relation": {}, "relation_version_is_last": true, "title": ["Dual-modality in vivo imaging of electroporation-mediated transgene expression in experimental tumors"], "weko_shared_id": -1}
Dual-modality in vivo imaging of electroporation-mediated transgene expression in experimental tumors
https://repo.qst.go.jp/records/69563
https://repo.qst.go.jp/records/6956344035f26-a5b6-49ec-8178-e318185e2cb3
Item type | 会議発表用資料 / Presentation(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2008-10-31 | |||||
タイトル | ||||||
タイトル | Dual-modality in vivo imaging of electroporation-mediated transgene expression in experimental tumors | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
資源タイプ | conference object | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
著者 |
U, Winn Aung
× U, Winn Aung× Hasegawa, Sumitaka× Koshikawa, Michiko× Furukawa, Takako× Saga, Tsuneo× U Winn Aung× 長谷川 純崇× 越川 道子× 古川 高子× 佐賀 恒夫 |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | In vivo electroporation (EP) is one of the efficient methods for effective gene transfer. Application of in vivo EP-mediated gene transfer (EGT) in anticancer gene therapy is highly expected since it could be performed repeatedly, safely and easily with low cost. Meanwhile, effective transfer, long-term expression and non-invasive monitoring are critical for optimal gene therapy. Here we report the feasibility of in vivo optical and MRI of EP-mediated gene expression in tumor model. Initially, we observed the in vivo EGT level and its temporal change by means of optical imaging using red fluorescence protein (RFP) as a reporter gene. Next, we constructed a dual reporter plasmid carrying a gene encoding ferritin heavy chain (FHC), a MRI reporter, and RFP gene to visualize transgene by dual modality. In cellular T2-weighted MRI, cells transfected with FHC plasmid showed lower signal intensity compared to the control cells. Moreover, after in vivo EGT, the plasmid-injected region in the tumor showed low signal intensity on T2-weighted MRI. Thus, our strategy would be a platform technology to evaluate EP-mediated gene therapy without necessity to administer contrast agent or substrate. | |||||
会議概要(会議名, 開催地, 会期, 主催者等) | ||||||
内容記述タイプ | Other | |||||
内容記述 | 第67回日本癌学会学術総会 | |||||
発表年月日 | ||||||
日付 | 2008-10-30 | |||||
日付タイプ | Issued |