量研学術機関リポジトリ「QST-Repository」は、国立研究開発法人 量子科学技術研究開発機構に所属する職員等が生み出した学術成果（学会誌発表論文、学会発表、研究開発報告書、特許等）を集積しインターネット上で広く公開するサービスです。 Welcome to QST-Repository where we accumulates and discloses the academic research results(Journal Publications, Conference presentation, Research and Development Report, Patent, etc.) of the members of National Institutes for Quantum Science and Technology.
Thank you very much for using our website. On the 11th of March 2019, this site was moved from our own network server to the JAIRO Cloud network server. If you previously bookmarked this site, that bookmark will no longer work. We would be grateful if you could bookmark the website again. Thank you very much for your understanding and cooperation.
In vivo biological effects induced by carbon-ion (C-ion) are still not fully understood. We examined gene expression changes for six mouse leg tumors of C3H/HeNrs mice using 44K oligo-microarrays at 6 hours (h), 1 day, and 3 days after C-ion irradiation with a single dose of 30 Gy. Gamma rays of 30 Gy and 50 Gy were used as a reference beam. In all six tumors, C-ion significantly changed the expression amounts of several tens of genes, including Cdkn1a, Ccng1, Ercc5, Mgmt, Cd80, and Polk at 6 h and 1 day, and Casp4, Tnfrsf6, Ikbke, Ifi202b, Fdps, and Ecm1 at 3 days. Expression changes of stress-responsive genes and cell communication-related genes lasted until 3 days. Expression changes of these genes in gamma-ray-treated samples were mostly similar or less than with C-ion, with large intertumor variance, while several genes, including Ikbke and Saa3, responded quite differentially from those irradiated by C-ions. Sequential immunohistochemical analysis of AurkA, Cdc20, and Cdkn1a revealed cell cycle/division arrest with mitotic catastrophe. Prolonged upregulation of stress-marker proteins and downregulation of cell-cycle promotion molecules provided evidence for the efficacy of this modality for local tumors.
マウスモデルにおける重粒子照射誘導ストレス反応遺伝子群の発現変化
