量研学術機関リポジトリ「QST-Repository」は、国立研究開発法人 量子科学技術研究開発機構に所属する職員等が生み出した学術成果（学会誌発表論文、学会発表、研究開発報告書、特許等）を集積しインターネット上で広く公開するサービスです。 Welcome to QST-Repository where we accumulates and discloses the academic research results(Journal Publications, Conference presentation, Research and Development Report, Patent, etc.) of the members of National Institutes for Quantum Science and Technology.
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To clarify the context of the gene-gene interrelationship in the manifestation of PIH, we analyzed Met235Thr variant of the angiotensinogen gene (AGT) and Glu298Asp variant of the endothelial nitric oxide synthase gene (NOS3) in terms of 4G/5G variant of the plasminogen activator inhibitor gene (PAI-1). One hundred forty-five Japanese patients with PIH were matched with 357 Japanese normal pregnant controls according to age and parity. Genotypings of variants of AGT, NOS3 and PAI-1 were carried out using PCR-RFLP methods after extraction of genomic DNA from 1.0 ml of whole blood samples. In 4G/4G of PAI-1, the frequency of T235 of AGT was higher in PIH (0.78) than in controls (0.56) (p<0.017), while in 4G/5G+5G/5G of PAI-1, no significant differences were found in the frequency of T235 of AGT between PIH (0.71) and controls (0.6). On the other hand, in 4G/4G of PAI-1, no significant differences were found in distributions of the frequency of GA+AA of NOS3 between PIH (0.18) and controls (0.1), but in 4G/5G+5G/5G of PAI-1, the frequency of GA+AA of NOS3 was higher in PIH (0.3) than in controls (0.15) (p<0.017). Women carrying T235 of AGT had an elevated risk for PIH because the local AGT expression is elevated in developing spiral arteries in women carrying this allele. On the other hand, Asp298 of NOS3 is associated with the reduction of NO in PIH. The present result suggests that coagulative tendency by 4G/4G of PAI-1 may play a role in pathogenesis of PIH in the subgroup possessing TT of AGT.
会議概要（会議名, 開催地, 会期, 主催者等）
The 56th Annual Meeting of The American Society of Human Genetics
A confoundings of candidate single nucleotide polmorphisms for pregnancy-induced hypertention in Japan.
