量研学術機関リポジトリ「QST-Repository」は、国立研究開発法人 量子科学技術研究開発機構に所属する職員等が生み出した学術成果（学会誌発表論文、学会発表、研究開発報告書、特許等）を集積しインターネット上で広く公開するサービスです。 Welcome to QST-Repository where we accumulates and discloses the academic research results(Journal Publications, Conference presentation, Research and Development Report, Patent, etc.) of the members of National Institutes for Quantum and Radiological Science and Technology.
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Mouse genome contains 1000 copies of intracisternal A-particle (IAP) DNA element that is closely related with endogeneous retrovirus. The IAP RNA is reversely transcribed and the cDNA is inserted into genomic DNA by the retrotransposition mechanism. Since functions of the gene at or nearby the novel insertion site of IAP cDNA are modified, the IAP element is presumable as endogenous mutagen. We found that genomic DNA rearrangement by the IAP-mediated retrotransposition is augmented in the myeloid leukemia cells in C3H/He inbred mouse.
Radiation-induced tumors are originated from cells in regenerated tissues after serious damages by ionizing radiation. In the regenerated myeloid cells following irradiation of sublethal doses at 3Gys of x-ray, the transcription level of a limited type among structural variants of the IAP elements in the genome were increased. Rearrangements of genomic DNA by unique insertions of the limited type of IAP cDNA are found in all the cell lines of acute myeloid leukemia induced by the radiation. This type of retrotransposition was observed in limited cell lines of radiation-induced thymic lymphoma and was hardly detected in the cells of non-hematopoietic tumor including skin carcinoma and hepatic tumors. Similar activation was not observed in the most types of IAP element. These findings indicate that an activation of the limited type of IAP element by radiation damage contributes to the genomic instability in the myeloid cells in C3H.
会議概要（会議名, 開催地, 会期, 主催者等）
The 2nd International Workshop on Space Radiation Research
Frequent Rearrangement of Genomic DNA by an Endogeneous Retrovirus in Radiation-induced Myeloid Leukemia Cells of C3H/He Inbred Mice
