量研学術機関リポジトリ「QST-Repository」は、国立研究開発法人 量子科学技術研究開発機構に所属する職員等が生み出した学術成果（学会誌発表論文、学会発表、研究開発報告書、特許等）を集積しインターネット上で広く公開するサービスです。 Welcome to QST-Repository where we accumulates and discloses the academic research results(Journal Publications, Conference presentation, Research and Development Report, Patent, etc.) of the members of National Institutes for Quantum and Radiological Science and Technology.
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Objective: [11C]DFMC was previously developed as a promising PET ligand for fatty acid amide hydrolase (FAAH), a key enzyme for regulation of endocannabinoid system in the central nervous system. Here, we aimed to quantify FAAH concentrations using PET with [11C]DFMC in brain regions.
Method: PET assessments with blood sampling were conducted for 90 min using rats. Kinetic analyses were performed by an irreversible two-tissue compartment model (2TCMi) and Patlak graphical analysis (PGA).
Results: The highest radioactive uptake of [11C]DFMC was obtained in the cingulate cortex. The kinetic parameters (λk3 and Ki) for FAAH concentrations in the cingulate cortex were 0.56 ± 0.08 and 0.19 ± 0.02 mL/cm3/min, respectively. The Ki values based on 2TCMi in all regions were similar to that of PGA, which supports that kinetic parameters based on compartment model were concisely estimated.
Conclusion: We successfully obtained in vivo mapping for regional FAAH concentrations by PET with [11C]DFMC.
Quantitative analysis for fatty acid amide hydrolase (FAAH) using PET with [11C]DFMC
