量研学術機関リポジトリ「QST-Repository」は、国立研究開発法人 量子科学技術研究開発機構に所属する職員等が生み出した学術成果（学会誌発表論文、学会発表、研究開発報告書、特許等）を集積しインターネット上で広く公開するサービスです。 Welcome to QST-Repository where we accumulates and discloses the academic research results(Journal Publications, Conference presentation, Research and Development Report, Patent, etc.) of the members of National Institutes for Quantum and Radiological Science and Technology.
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Aim of this study is to understand cellular and molecular nature of cancer cells survived for long term after charged particle exposure. During the period of 1st year, clonogenic sensitivity of various cancer cell lines against charged particles was investigated by two experimental strategies. Firstly, mouse cancer cell line analysis in the presence of 5-azacytidine, a DNA methylation inhibitor, revealed epigenetic heterogeneity of charged particle sensitivity within the cell population. In the second approach, human glioblastoma cell line, Becker, was investigated for the phenotypic changes after long term survival period (3 weeks). Especially, the cells were revealed to be sensitized toward secondary exposure of charged particles in a way of primary dose-dependence. However, this tendency was clearly eliminated when cells were treated by 5-azacytidine before the primary exposure. Thus, epigenetic regulations of cellular genomic DNA were also supposed to play important roles in the radiation sensitivity changes of the long-term survived cells.
重粒子線照射によるゲノム構造と遺伝子発現量の変化
