量研学術機関リポジトリ「QST-Repository」は、国立研究開発法人 量子科学技術研究開発機構に所属する職員等が生み出した学術成果（学会誌発表論文、学会発表、研究開発報告書、特許等）を集積しインターネット上で広く公開するサービスです。 Welcome to QST-Repository where we accumulates and discloses the academic research results(Journal Publications, Conference presentation, Research and Development Report, Patent, etc.) of the members of National Institutes for Quantum Science and Technology.
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X-ray induces myeloid leukemia in C3H/He mice with the incidence of 20-25% at 3Gy. Mutation of Sfpi1 (PU.1) is crucial in the development of myeloid leukemia. The mutation, however, is mostly specific point mutations in the DNA-binding Ets domain of PU.1, and they are unlikely to be a direct consequence of radiation-induced DNA damage. To elucidate when and how often this type of mutation arises in hematopoietic cells, we have developed a technique that is capable of detecting a small number of mutated cells in the vast majority of non-mutated cells. The technique is based on the wild-type blocking PCR, in which LNA (locked nucleic acid) oligonucleotide blocks amplification of wild-type DNA during PCR while permitting amplification of mutants. The PCR followed by restriction enzyme digestion has enabled to detect mutant DNA in 104 times amount of wild-type DNA. This technique is being applied to DNA samples from C3H/He mice that were exposed to 3Gy of x-rays and were maintained for more than a year. Among 12 exposed mice and 6 age-matched controls analyzed so far, the mutation has been detected in a sample from the spleen of an exposed mouse.
Detection of Leukemia-Specific Mutations in Mice Exposed to Radiation
