量研学術機関リポジトリ「QST-Repository」は、国立研究開発法人 量子科学技術研究開発機構に所属する職員等が生み出した学術成果（学会誌発表論文、学会発表、研究開発報告書、特許等）を集積しインターネット上で広く公開するサービスです。 Welcome to QST-Repository where we accumulates and discloses the academic research results(Journal Publications, Conference presentation, Research and Development Report, Patent, etc.) of the members of National Institutes for Quantum Science and Technology.
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Purpose---To clarify the molecular mechanism of radiation induced late
injury in normal tissue, using murine model. In the first year of this
study, aiming to irradiate the lung or the urinary bladder of mice with
carbon-ion, new type of collimators was constructed.
Materials and methods --- C3H/HeNrs and C57BL/6J, 8-week-old female mice
were used through experiments. The position of the target, either lung or
bladder, was confirmed by x-ray photographs. Also, the absorbed dose at the
target was confirmed using photoluminescence dosimeter (PLD) and ionization
chamber. Exposures were conducted using horizontal carbon-12 beams with 290
MeV/u at a dose rate of approximately 3 Gy/min within the 6-cm spread-out
bragg peak (SOBP). Gamma-rays were used as reference beam with new
collimators. To evaluate the effects of C-ion irradiation on lungs,
pathological changes and immunohistochemical stainings for CD44 and Bak at 6
hr, day16, 28 and 56 after thoracic irradiation were analyzed. To evaluate
the adverse effects on the bladder,cystometry and pathological evaluation
were performed.The total RNA was extracted from irradiated tissues, which
were removed on day1,4,8,15,29,60,90,150,240 and 360 after irradiation for
microarray analysis to elucidate the molecular response to irradiation.
Results --- The number of CD44 and Bak positive cells in the irradiated lungs
were changed after irradiation. The capacity of the urinary bladder and the
compliance were decreased significantly at day1,4,8 and 29 after
irradiation.
晩期障害マウスモデルを用いた重粒子線治療有害事象の検討
