WEKO3
アイテム
{"_buckets": {"deposit": "d5b1e5b7-b3cf-4e81-86ae-225f4cf41990"}, "_deposit": {"created_by": 1, "id": "61026", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "61026"}, "status": "published"}, "_oai": {"id": "oai:repo.qst.go.jp:00061026", "sets": ["29"]}, "author_link": ["604819", "604818", "604821", "604825", "604831", "604830", "604816", "604812", "604811", "604815", "604835", "604828", "604827", "604840", "604824", "604814", "604836", "604817", "604822", "604829", "604832", "604820", "604837", "604839", "604833", "604823", "604813", "604834", "604838", "604826"], "item_10005_date_7": {"attribute_name": "発表年月日", "attribute_value_mlt": [{"subitem_date_issued_datetime": "2004-10-30", "subitem_date_issued_type": "Issued"}]}, "item_10005_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "During the course of studying the genetic determinants of normal tissue injury after radiotherapy of cancer patients, we have detected positive correlation of some intronic SNPs on human DNA ligase I locus of chromosome 19 to the severity of the injury. The human DNA ligase I gene has three consecutive GT-repeat sequences franked by the same polypurine-rich sequence at a place very close to the exon-intron boundary of an alternatively spliced intron. In this study, we have analyzed stability of the GT-repeat sequences among individuals and haplotype structure surrounding this locus.\nThe genomic region containing the above sequences were amplified from the blood DNA of 9 cancer patients by PCR and cloned into TA-cloning vector. Plasmid DNAs from 6 independent colonies of each transformant were purified and sequenced, respectively. We observed variations of the all three GT-repeats among cancer patients and also among the clones from each individual. Haplotype structure of the 4 SNPs that surround the GT-repeat sequences was estimated using the EM algorism. The nine cancer individuals were divided into 3 groups according to the above estimated haplotypes. By comparing the haplotype structure and the GT-repeat sequences, we found that the GT-repeat sequence that located closest to the exon-intron boundary seemed to have solid threshold range in variation for each haplotype.\nFunctional relevance of the human DNA ligase I gene haplotype with different threshold range in length variation of GT-repeat sequence, in a sense related to the individual\u0027s radiation sensitivity, will be elucidated in further study.", "subitem_description_type": "Abstract"}]}, "item_10005_description_6": {"attribute_name": "会議概要(会議名, 開催地, 会期, 主催者等)", "attribute_value_mlt": [{"subitem_description": "ASHG Meeting 2004", "subitem_description_type": "Other"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "metadata only access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_14cb"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Michikawa, Yuichi"}], "nameIdentifiers": [{"nameIdentifier": "604811", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Suga, Tomo"}], "nameIdentifiers": [{"nameIdentifier": "604812", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kouda, Masakazu"}], "nameIdentifiers": [{"nameIdentifier": "604813", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ootsuka, Yoshimi"}], "nameIdentifiers": [{"nameIdentifier": "604814", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Suzuki, Hideyuki"}], "nameIdentifiers": [{"nameIdentifier": "604815", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Saegusa, Kumiko"}], "nameIdentifiers": [{"nameIdentifier": "604816", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Furuno, Aki"}], "nameIdentifiers": [{"nameIdentifier": "604817", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ishikawa, Ken-ichi"}], "nameIdentifiers": [{"nameIdentifier": "604818", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ishikawa, Atsuko"}], "nameIdentifiers": [{"nameIdentifier": "604819", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kawai, Seiko"}], "nameIdentifiers": [{"nameIdentifier": "604820", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Tsuji, Atsushi"}], "nameIdentifiers": [{"nameIdentifier": "604821", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sugyou, Aya"}], "nameIdentifiers": [{"nameIdentifier": "604822", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Noda, Shuhei"}], "nameIdentifiers": [{"nameIdentifier": "604823", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Iwakawa, Mayumi"}], "nameIdentifiers": [{"nameIdentifier": "604824", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Imai, Takashi"}], "nameIdentifiers": [{"nameIdentifier": "604825", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "道川 祐市", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "604826", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "菅 智", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "604827", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "神田 将和", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "604828", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "荘司 好美", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "604829", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "鈴木 英幸", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "604830", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "三枝 公美子", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "604831", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "古野 亜紀", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "604832", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "石川 顕一", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "604833", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "石川 敦子", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "604834", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "川井 聖子", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "604835", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "辻 厚至", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "604836", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "須尭 綾", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "604837", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "野田 秀平", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "604838", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "岩川 眞由美", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "604839", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "今井 高志", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "604840", "nameIdentifierScheme": "WEKO"}]}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "conference object", "resourceuri": "http://purl.org/coar/resource_type/c_c94f"}]}, "item_title": "Variation of GT-repeat numbers associated with haplotype structure of human DNA ligase I locus", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Variation of GT-repeat numbers associated with haplotype structure of human DNA ligase I locus"}]}, "item_type_id": "10005", "owner": "1", "path": ["29"], "permalink_uri": "https://repo.qst.go.jp/records/61026", "pubdate": {"attribute_name": "公開日", "attribute_value": "2005-07-07"}, "publish_date": "2005-07-07", "publish_status": "0", "recid": "61026", "relation": {}, "relation_version_is_last": true, "title": ["Variation of GT-repeat numbers associated with haplotype structure of human DNA ligase I locus"], "weko_shared_id": -1}
Variation of GT-repeat numbers associated with haplotype structure of human DNA ligase I locus
https://repo.qst.go.jp/records/61026
https://repo.qst.go.jp/records/6102634c6031f-d972-4fcf-b804-f5d55c10af64
Item type | 会議発表用資料 / Presentation(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2005-07-07 | |||||
タイトル | ||||||
タイトル | Variation of GT-repeat numbers associated with haplotype structure of human DNA ligase I locus | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
資源タイプ | conference object | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
著者 |
Michikawa, Yuichi
× Michikawa, Yuichi× Suga, Tomo× Kouda, Masakazu× Ootsuka, Yoshimi× Suzuki, Hideyuki× Saegusa, Kumiko× Furuno, Aki× Ishikawa, Ken-ichi× Ishikawa, Atsuko× Kawai, Seiko× Tsuji, Atsushi× Sugyou, Aya× Noda, Shuhei× Iwakawa, Mayumi× Imai, Takashi× 道川 祐市× 菅 智× 神田 将和× 荘司 好美× 鈴木 英幸× 三枝 公美子× 古野 亜紀× 石川 顕一× 石川 敦子× 川井 聖子× 辻 厚至× 須尭 綾× 野田 秀平× 岩川 眞由美× 今井 高志 |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | During the course of studying the genetic determinants of normal tissue injury after radiotherapy of cancer patients, we have detected positive correlation of some intronic SNPs on human DNA ligase I locus of chromosome 19 to the severity of the injury. The human DNA ligase I gene has three consecutive GT-repeat sequences franked by the same polypurine-rich sequence at a place very close to the exon-intron boundary of an alternatively spliced intron. In this study, we have analyzed stability of the GT-repeat sequences among individuals and haplotype structure surrounding this locus. The genomic region containing the above sequences were amplified from the blood DNA of 9 cancer patients by PCR and cloned into TA-cloning vector. Plasmid DNAs from 6 independent colonies of each transformant were purified and sequenced, respectively. We observed variations of the all three GT-repeats among cancer patients and also among the clones from each individual. Haplotype structure of the 4 SNPs that surround the GT-repeat sequences was estimated using the EM algorism. The nine cancer individuals were divided into 3 groups according to the above estimated haplotypes. By comparing the haplotype structure and the GT-repeat sequences, we found that the GT-repeat sequence that located closest to the exon-intron boundary seemed to have solid threshold range in variation for each haplotype. Functional relevance of the human DNA ligase I gene haplotype with different threshold range in length variation of GT-repeat sequence, in a sense related to the individual's radiation sensitivity, will be elucidated in further study. |
|||||
会議概要(会議名, 開催地, 会期, 主催者等) | ||||||
内容記述タイプ | Other | |||||
内容記述 | ASHG Meeting 2004 | |||||
発表年月日 | ||||||
日付 | 2004-10-30 | |||||
日付タイプ | Issued |