量研学術機関リポジトリ「QST-Repository」は、国立研究開発法人 量子科学技術研究開発機構に所属する職員等が生み出した学術成果（学会誌発表論文、学会発表、研究開発報告書、特許等）を集積しインターネット上で広く公開するサービスです。 Welcome to QST-Repository where we accumulates and discloses the academic research results(Journal Publications, Conference presentation, Research and Development Report, Patent, etc.) of the members of National Institutes for Quantum Science and Technology.
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Understanding the individuals variation in radiation sensitivity at a molecular level may be essential to predict unusual response of normal tissue cells to clinical radiations. Micro array technology has been proved to be a powerful tool for comparing gene expression in normal and disease states and/or for analyzing the response of cells exposed to drugs or unknown physiological conditions. We have studied gene expression profiles of the cultured neonatal human dermal fibroblasts (NHDF) using the custom-made oligonucleotide microarray, containing 22,500 probes (representing 19,862unique genes), before or/after exposure to 0.1, 2 and 5Gy of X rays and carbon-ion beams. Total RNA was purified from the cells at 1, 3, 6, 12, 18, 24 hours after irradiations. The expression level of some genes was confirmed with a quantative reverse transcription-PCR.
ヒト培養細胞を用いた重粒子線照射における遺伝子発現変化の検討
