量研学術機関リポジトリ「QST-Repository」は、国立研究開発法人 量子科学技術研究開発機構に所属する職員等が生み出した学術成果（学会誌発表論文、学会発表、研究開発報告書、特許等）を集積しインターネット上で広く公開するサービスです。 Welcome to QST-Repository where we accumulates and discloses the academic research results(Journal Publications, Conference presentation, Research and Development Report, Patent, etc.) of the members of National Institutes for Quantum Science and Technology.
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γ-Glutamyl transferase (GGT) is membrane-bound enzyme that catalyzes the cleavage of γ-glutamyl moiety of extracellular glutathione to supply cysteine for intracellular synthesis of glutathione as anti-oxidant. Elevated GGT activity has been found in wide range of cancers, where GGT promotes tumor cell survival, tumor progression, and resistance to radiotherapy and chemotherapies. Based on biological and clinical studies, we hypothesize that non-invasive methods for real-time monitoring of GGT activity will provide the information of cancer characterization, prognosis, and development of therapeutic strategy.
Hyperpolarized (HP) 13C Magnetic Resonance spectroscopy (MRS) and imaging which have been newly developed modality enable non-invasive molecular imaging of real-time metabolism in tumor. Despite of the advantageous feature, only a limited number of 13C-labeled probes have been utilized for in vivo applications because of their physicochemical property. Dr. Nishihara et al., however, have successfully designed and prepared a novel HP molecular probe for GGT activity, γ-Glu-[1-13C]Gly. γ-Glu-[1-13C]Gly was designed to produce a distinct 13C signal in chemical shift range by the enzymatic cleavage, due to the positively charged amino group of released [1-13C]Gly in physiological pH. It was demonstrated that this probe can monitor real-time GGT activity in vivo rat kidney as well as in vitro homogenate samples.
Herein, we investigated to apply this novel probe with HP-MRS technology to in vivo tumor xenograft.
雑誌名
ISMRM 2018 proceedings
ページ
3052
発行年
2018-06
出版者
International Society for Magnetic Resonance in Medicine
Application of a novel 13C hyperpolarized metabolic tracer for gamma-Glutamyl transferase activity in vivo tumor xenograft
