量研学術機関リポジトリ「QST-Repository」は、国立研究開発法人 量子科学技術研究開発機構に所属する職員等が生み出した学術成果（学会誌発表論文、学会発表、研究開発報告書、特許等）を集積しインターネット上で広く公開するサービスです。 Welcome to QST-Repository where we accumulates and discloses the academic research results(Journal Publications, Conference presentation, Research and Development Report, Patent, etc.) of the members of National Institutes for Quantum Science and Technology.
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In present study we describe in vitro imaging analysis of cellular redox status in cancer and noncancer cells, using two redox-sensitive nitroxide derivatives (methoxy-TEMPO and mito-TEMPO) and electron paramagnetic resonance (EPR) spectroscopy. The nitroxide radical (which is characterized by EPR contrast) participates in electron-transfer reactions with variety of intracellular oxidative and reducing equivalents. The rate constants of these reactions determine the EPR signal dynamics in cell suspension. In non-cancer cells, EPR signal intensity of nitroxides decreased significantly during incubation. In cancer cells, EPR signal intensity was almost constant within two hours incubation and did not decreased significantly. The data suggest that non-cancer cells are characterized by a higher reducing activity to the nitroxide radical, while cancer cells are characterized by a higher oxidative activity. The described methodology is appropriate sensing platform for differentiation of cancer from non-cancer cells based on their redox status.
雑誌名
Anticancer Research
巻
34
ページ
5917 - 5919
発行年
2014-10
出版者
International Institute of Anticancer Research (IIAR)
In vitro analysis of redox status of normal and tumor cells using nitroxide radicals and EPR spectroscopy
