量研学術機関リポジトリ「QST-Repository」は、国立研究開発法人 量子科学技術研究開発機構に所属する職員等が生み出した学術成果（学会誌発表論文、学会発表、研究開発報告書、特許等）を集積しインターネット上で広く公開するサービスです。 Welcome to QST-Repository where we accumulates and discloses the academic research results(Journal Publications, Conference presentation, Research and Development Report, Patent, etc.) of the members of National Institutes for Quantum Science and Technology.
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Chordoma, a rare bone tumor, has been treated with surgery and/or radiation. However, only limited characterizations of chordoma cells are available due to the extremely long doubling time. To study the cell biology of chordoma, we derived and characterized a cell line with a shorter doubling time from the only available chordoma line U-CH1. After isolating a clone of U-CH1 cells with a short doubling time (U-CH1-N), cell growth, cell cycle distribution, DNA content, chromosome number, p53 status, and cell survival were examined after X-rays, heavy ions, camptothecin, mitomycin C, cisplatin and bleomycin. These data were compared with those of HeLa (cervical cancer) and U87-MG (glioblastoma) cells. The cell doubling times for HeLa, U87-MG and U-CH1-N were approximately 18 h, 24 h and 3 days respectively. Heavy ion irradiation killed all three lines more efficiently than x-rays. Relative biological effectiveness (RBE) at 10% survival for UCH-1-N was about 2.5 for 70keV/microm carbon and 4 for 200keV/microm iron ions. Among four chemicals, bleomycin showed the most marked cytotoxic effect on U-CH1-N. Our data provide the first chronological cell survival information using cells of chordoma origin and help explain the successful chordoma treatment by heavy ions.
雑誌名
National Institute of Radiological Sciences Annual Report
Radiobiology of Chordoma Cells
