WEKO3
アイテム
{"_buckets": {"deposit": "0eb71849-4e38-4ce8-b1b4-f8853f2648ac"}, "_deposit": {"created_by": 1, "id": "49445", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "49445"}, "status": "published"}, "_oai": {"id": "oai:repo.qst.go.jp:00049445", "sets": ["1"]}, "author_link": ["499373", "499376", "499378", "499374", "499379", "499377", "499375", "499372"], "item_8_biblio_info_7": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2018-08", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "14", "bibliographicPageEnd": "7137", "bibliographicPageStart": "7124", "bibliographicVolumeNumber": "46", "bibliographic_titles": [{"bibliographic_title": "Nucleic Acids Research"}]}]}, "item_8_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "The first nucleosome in the downstream of transcription start site is called +1 nucleosome, which is expected to be readily unwrapped for DNA transcription. To investigate the DNA accessibility of +1 nucleosomes, MNase-seq experiments were carried out with 20 reconstituted +1 nucleosomes of yeast. We find that MNase not only cleaves the presumably accessible end regions but also the further inward sites where AA/TT dinucleotide is abundant. Since AA/TT is known as a rigid dinucleotide step resistant to DNA bending, these internal cleavages reflect the local site exposures induced by the physicochemical nature of DNA sequence. As the DNA entry site of +1 nucleosomes in yeast is found AA/TT-rich, we propose that this sequence element might be utilized by gene regulation to lower DNA-histones affinity to facilitate DNA unwrapping for transcription. Accordingly, our further analysis shows that the highly transcribed genes in yeast are more likely to position more AA/TTs in the entry-to-dyad region than the exit-to-dyad region in their +1 nucleosomes.", "subitem_description_type": "Abstract"}]}, "item_8_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "10.1093/nar/gky502", "subitem_relation_type_select": "DOI"}}]}, "item_8_relation_17": {"attribute_name": "関連サイト", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "https://doi.org/10.1093/nar/gky502"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1093/nar/gky502", "subitem_relation_type_select": "DOI"}}]}, "item_8_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0305-1048", "subitem_source_identifier_type": "ISSN"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "metadata only access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_14cb"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Luo, Di"}], "nameIdentifiers": [{"nameIdentifier": "499372", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kato, Daiki"}], "nameIdentifiers": [{"nameIdentifier": "499373", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nogami, Jumpei"}], "nameIdentifiers": [{"nameIdentifier": "499374", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ohkawa, Yasuyuki"}], "nameIdentifiers": [{"nameIdentifier": "499375", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kurumizaka, Hitoshi"}], "nameIdentifiers": [{"nameIdentifier": "499376", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kono, Hidetoshi"}], "nameIdentifiers": [{"nameIdentifier": "499377", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "ルオ ディ", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "499378", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "河野 秀俊", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "499379", "nameIdentifierScheme": "WEKO"}]}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "MNase, as a probe to study the sequence-dependent site exposures in the +1 nucleosomes of yeast", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "MNase, as a probe to study the sequence-dependent site exposures in the +1 nucleosomes of yeast"}]}, "item_type_id": "8", "owner": "1", "path": ["1"], "permalink_uri": "https://repo.qst.go.jp/records/49445", "pubdate": {"attribute_name": "公開日", "attribute_value": "2019-01-10"}, "publish_date": "2019-01-10", "publish_status": "0", "recid": "49445", "relation": {}, "relation_version_is_last": true, "title": ["MNase, as a probe to study the sequence-dependent site exposures in the +1 nucleosomes of yeast"], "weko_shared_id": -1}
MNase, as a probe to study the sequence-dependent site exposures in the +1 nucleosomes of yeast
https://repo.qst.go.jp/records/49445
https://repo.qst.go.jp/records/494454536182a-7a21-4c24-9618-0ec792c20b81
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2019-01-10 | |||||
タイトル | ||||||
タイトル | MNase, as a probe to study the sequence-dependent site exposures in the +1 nucleosomes of yeast | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
著者 |
Luo, Di
× Luo, Di× Kato, Daiki× Nogami, Jumpei× Ohkawa, Yasuyuki× Kurumizaka, Hitoshi× Kono, Hidetoshi× ルオ ディ× 河野 秀俊 |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The first nucleosome in the downstream of transcription start site is called +1 nucleosome, which is expected to be readily unwrapped for DNA transcription. To investigate the DNA accessibility of +1 nucleosomes, MNase-seq experiments were carried out with 20 reconstituted +1 nucleosomes of yeast. We find that MNase not only cleaves the presumably accessible end regions but also the further inward sites where AA/TT dinucleotide is abundant. Since AA/TT is known as a rigid dinucleotide step resistant to DNA bending, these internal cleavages reflect the local site exposures induced by the physicochemical nature of DNA sequence. As the DNA entry site of +1 nucleosomes in yeast is found AA/TT-rich, we propose that this sequence element might be utilized by gene regulation to lower DNA-histones affinity to facilitate DNA unwrapping for transcription. Accordingly, our further analysis shows that the highly transcribed genes in yeast are more likely to position more AA/TTs in the entry-to-dyad region than the exit-to-dyad region in their +1 nucleosomes. | |||||
書誌情報 |
Nucleic Acids Research 巻 46, 号 14, p. 7124-7137, 発行日 2018-08 |
|||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0305-1048 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1093/nar/gky502 | |||||
関連サイト | ||||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1093/nar/gky502 | |||||
関連名称 | https://doi.org/10.1093/nar/gky502 |