量研学術機関リポジトリ「QST-Repository」は、国立研究開発法人 量子科学技術研究開発機構に所属する職員等が生み出した学術成果（学会誌発表論文、学会発表、研究開発報告書、特許等）を集積しインターネット上で広く公開するサービスです。 Welcome to QST-Repository where we accumulates and discloses the academic research results(Journal Publications, Conference presentation, Research and Development Report, Patent, etc.) of the members of National Institutes for Quantum Science and Technology.
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In biological dosimetry by counting chromosome aberrations at low dose radiation exposure, automation technique has been required to process large number of sample preparations. The metaphase finder is an automated optical microscope system, which automatically scans and finds metaphase cells on the slide glass in low magnification, and relocates metaphase cells to the center of the field of view of the microscope to observe chromosomes in high magnification. The authors have constructed a cost-effective metaphase finder system by assembling commercially available components, such as microscope, motorized sample stage, personal computer and general purpose image analysis software package, instead of purchasing one dedicated system. The new system has high cost-effectiveness and high flexibility in adapting to the new staining methods. The mathematical morphology based image processing to the algorithm was used to find metaphase cells. Especially, the morphology for the gray-image was used for the newest version. The performance of this metaphase finder system was tested. The new machines were distributed to six institutes of The Chromosome Network for biodosimetry in Japan for the testing the new algorithm for the practical use. The scanning speed was 14.5 to 18.1 minutes per square centimeter. It was slower than several commercial metaphase finders but false positive were enough small. This system is economical and satisfactory for practical use.
Low-cost metaphase finder system
