量研学術機関リポジトリ「QST-Repository」は、国立研究開発法人 量子科学技術研究開発機構に所属する職員等が生み出した学術成果（学会誌発表論文、学会発表、研究開発報告書、特許等）を集積しインターネット上で広く公開するサービスです。 Welcome to QST-Repository where we accumulates and discloses the academic research results(Journal Publications, Conference presentation, Research and Development Report, Patent, etc.) of the members of National Institutes for Quantum and Radiological Science and Technology.
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In the present study, we examined copper accumulation and distribution in the gills of Japanese freshwater crabs Geothelphusa dehaani exposed to copper chloride, using microbeam scanning particle induced X-ray emission (micro-PIXE) analysis to clarify the potential of this decapod crustacean as an indicator organism for copper pollution. After exposure of the crabs to copper chloride (1 mg/L) for 3 days, the copper concentration in the gills was 161 ± 48 μg/g of tissue wet weight, which was four times higher than the control value. Copper was found not only on the surface, but also inside the gills, and especially localized at the gill axis. In the morphological examination, severe damage of the gills was not observed in the exposure group, while hemagglutination was detected at the gill axis. The results suggest that the Japanese freshwater crab would be useful as a model organism for understanding mechanisms of toxicity, and that crab gills may be an effective indicator of copper pollution.
マイクロPIXE 分析を用いたサワガニ（Geothelphusa dehaani）の鰓における銅の分布解析
