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Tumorbearing\nmice received 5-bromo-2’-deoxyuridine (BrdU) continuously\nto label all intratumor proliferating (P) cells. They received γ-rays or\naccelerated carbon-ion beams at a high or reduced dose-rate. Other\ntumor-bearing mice received reactor thermal or epithermal neutrons\nat a reduced dose-rate. Immediately or 9 hours after the high doserate\nirradiation (HDRI), or immediately after the reduced dose-rate\nirradiation (RDRI), the tumor cells were isolated and incubated with\na cytokinesis blocker, and the micronucleus (MN) frequency in cells\nwithout BrdU labeling (Q cells) was determined using immunofluorescence\nstaining for BrdU.\nResults: The difference in radiosensitivity between the total (P + Q)\nand Q cells after γ-ray irradiation was markedly reduced with reactor\nneutron beams or carbon-ion beams, especially with a higher linear\nenergy transfer (LET) value. Following γ-ray irradiation, SAS/neo\ntumor cells, especially intratumor Q cells, showed a marked reduction\nin sensitivity due to the recovery from radiation-induced damage,\ncompared with the total or Q cells within SAS/mp53 tumors that\nshowed little repair capacity. In both total and Q cells within both\nSAS/neo and SAS/mp53 tumors, carbon-ion beam irradiation, especially\nwith a higher LET, showed little recovery capacity through\nleaving an interval between HDRI and the assay or decreasing the\ndose-rate. The recovery from radiation-induced damage after γ-ray\nirradiation was a p53-dependent event, but little recovery was found\nafter carbon-ion beam irradiation. With RDRI, the radiosensitivity\nto reactor thermal and epithermal neutron beams was slightly higher\nthan that to carbon-ion beams.\nConclusion: For tumor control, including intratumor Q-cell control,\naccelerated carbon-ion beams, especially with a higher LET, and reactor\nthermal and epithermal neutron beams were very useful for suppressing\nthe recovery from radiation-induced damage irrespective of\np53 status of tumor cells.", "subitem_description_type": "Abstract"}]}, "item_8_publisher_8": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "World J Oncol and Elmer Press Inc"}]}, "item_8_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "http://dx.doi.org/10.14740/wjon941w", "subitem_relation_type_select": "DOI"}}]}, "item_8_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1920-454X", "subitem_source_identifier_type": "ISSN"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "metadata only access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_14cb"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "増永, 慎一郎"}], "nameIdentifiers": [{"nameIdentifier": "475464", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "鵜澤, 玲子"}], "nameIdentifiers": [{"nameIdentifier": "475465", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "平山, 亮一"}], "nameIdentifiers": [{"nameIdentifier": "475466", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "松本, 孔貴"}], "nameIdentifiers": [{"nameIdentifier": "475467", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sakurai, Yoshinori"}], "nameIdentifiers": [{"nameIdentifier": "475468", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "田中, 浩基"}], "nameIdentifiers": [{"nameIdentifier": "475469", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Tano, Keizo"}], "nameIdentifiers": [{"nameIdentifier": "475470", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sanada, Yu"}], "nameIdentifiers": [{"nameIdentifier": "475471", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Suzuki, Minoru"}], "nameIdentifiers": [{"nameIdentifier": "475472", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "丸橋, 晃"}], "nameIdentifiers": [{"nameIdentifier": "475473", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "小野, 公二"}], "nameIdentifiers": [{"nameIdentifier": "475474", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "増永 慎一郎", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "475475", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "鵜澤 玲子", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "475476", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "平山 亮一", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "475477", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "松本 孔貴", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "475478", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "田中 浩基", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "475479", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "丸橋 晃", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "475480", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "小野 公二", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "475481", "nameIdentifierScheme": "WEKO"}]}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "The Effect of p53 Status of Tumor Cells on Radiosensitivity of Irradiated Tumors With Carbon-Ion Beams Compared With γ-Rays or Reactor Neutron Beams", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "The Effect of p53 Status of Tumor Cells on Radiosensitivity of Irradiated Tumors With Carbon-Ion Beams Compared With γ-Rays or Reactor Neutron Beams"}]}, "item_type_id": "8", "owner": "1", "path": ["1"], "permalink_uri": "https://repo.qst.go.jp/records/47462", "pubdate": {"attribute_name": "公開日", "attribute_value": "2016-08-10"}, "publish_date": "2016-08-10", "publish_status": "0", "recid": "47462", "relation": {}, "relation_version_is_last": true, "title": ["The Effect of p53 Status of Tumor Cells on Radiosensitivity of Irradiated Tumors With Carbon-Ion Beams Compared With γ-Rays or Reactor Neutron Beams"], "weko_shared_id": -1}
The Effect of p53 Status of Tumor Cells on Radiosensitivity of Irradiated Tumors With Carbon-Ion Beams Compared With γ-Rays or Reactor Neutron Beams
https://repo.qst.go.jp/records/47462
https://repo.qst.go.jp/records/474626af78363-339a-46f6-afae-750a91a310a2
Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2016-08-10 | |||||
タイトル | ||||||
タイトル | The Effect of p53 Status of Tumor Cells on Radiosensitivity of Irradiated Tumors With Carbon-Ion Beams Compared With γ-Rays or Reactor Neutron Beams | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
著者 |
増永, 慎一郎
× 増永, 慎一郎× 鵜澤, 玲子× 平山, 亮一× 松本, 孔貴× Sakurai, Yoshinori× 田中, 浩基× Tano, Keizo× Sanada, Yu× Suzuki, Minoru× 丸橋, 晃× 小野, 公二× 増永 慎一郎× 鵜澤 玲子× 平山 亮一× 松本 孔貴× 田中 浩基× 丸橋 晃× 小野 公二 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Background: The aim of the study was to clarify the effect of p53 status of tumor cells on radiosensitivity of solid tumors following accelerated carbon-ion beam irradiation compared with γ-rays or reactor neutron beams, referring to the response of intratumor quiescent (Q) cells. Methods: Human head and neck squamous cell carcinoma cells transfected with mutant TP53 (SAS/mp53) or with neo vector (SAS/neo) were injected subcutaneously into hind legs of nude mice. Tumorbearing mice received 5-bromo-2’-deoxyuridine (BrdU) continuously to label all intratumor proliferating (P) cells. They received γ-rays or accelerated carbon-ion beams at a high or reduced dose-rate. Other tumor-bearing mice received reactor thermal or epithermal neutrons at a reduced dose-rate. Immediately or 9 hours after the high doserate irradiation (HDRI), or immediately after the reduced dose-rate irradiation (RDRI), the tumor cells were isolated and incubated with a cytokinesis blocker, and the micronucleus (MN) frequency in cells without BrdU labeling (Q cells) was determined using immunofluorescence staining for BrdU. Results: The difference in radiosensitivity between the total (P + Q) and Q cells after γ-ray irradiation was markedly reduced with reactor neutron beams or carbon-ion beams, especially with a higher linear energy transfer (LET) value. Following γ-ray irradiation, SAS/neo tumor cells, especially intratumor Q cells, showed a marked reduction in sensitivity due to the recovery from radiation-induced damage, compared with the total or Q cells within SAS/mp53 tumors that showed little repair capacity. In both total and Q cells within both SAS/neo and SAS/mp53 tumors, carbon-ion beam irradiation, especially with a higher LET, showed little recovery capacity through leaving an interval between HDRI and the assay or decreasing the dose-rate. The recovery from radiation-induced damage after γ-ray irradiation was a p53-dependent event, but little recovery was found after carbon-ion beam irradiation. With RDRI, the radiosensitivity to reactor thermal and epithermal neutron beams was slightly higher than that to carbon-ion beams. Conclusion: For tumor control, including intratumor Q-cell control, accelerated carbon-ion beams, especially with a higher LET, and reactor thermal and epithermal neutron beams were very useful for suppressing the recovery from radiation-induced damage irrespective of p53 status of tumor cells. |
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書誌情報 |
World Journal of Oncology 巻 6, 号 4, p. 398-409, 発行日 2015-09 |
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出版者 | ||||||
出版者 | World J Oncol and Elmer Press Inc | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1920-454X | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | http://dx.doi.org/10.14740/wjon941w |