WEKO3
アイテム
{"_buckets": {"deposit": "d6510cb4-0bce-4f21-9b5f-b989df8eaf00"}, "_deposit": {"created_by": 1, "id": "47288", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "47288"}, "status": "published"}, "_oai": {"id": "oai:repo.qst.go.jp:00047288", "sets": ["1"]}, "author_link": ["472981", "472970", "472977", "472978", "472979", "472975", "472972", "472974", "472973", "472980", "472971", "472976"], "item_8_biblio_info_7": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2015-06", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "4", "bibliographicPageStart": "e00228", "bibliographicVolumeNumber": "35", "bibliographic_titles": [{"bibliographic_title": "Bioscience Reports"}]}]}, "item_8_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "The BCNT (Bucentaur) superfamily is classified by an uncharacteristic conserved sequence of ∼80 amino acids (aa)\nat the C-terminus, BCNT-C (the conserved C-terminal region of Bcnt/Cfdp1). Whereas the yeast Swc5 and Drosophila\nYeti homologues play crucial roles in chromatin remodelling organization, mammalian Bcnt/Cfdp1 (craniofacial\ndevelopmental protein 1) remains poorly understood. The protein, which lacks cysteine, is largely disordered and\ncomprises an acidic N-terminal region, a lysine/glutamic acid/proline-rich 40 aa sequence and BCNT-C. It shows\ncomplex mobility on SDS/PAGE at ∼50 kDa, whereas its calculated molecular mass is ∼33 kDa. To characterize this\nmobility discrepancy and the effects of post-translational modifications (PTMs), we expressed various deleted His–\nBcnt in E. coli and HEK cells and found that an acidic stretch in the N-terminal region is a main cause of the gel shift.\nExogenous BCNT/CFDP1 constitutively expressed in HEK clones appears as a doublet at 49 and 47 kDa, slower than\nthe protein expressed in Escherichia coli but faster than the endogenous protein on SDS/PAGE. Among seven in vivo\nphosphorylation sites, Ser250, which resides in a region between disordered and ordered regions in BCNT-C, is heavily\nphosphorylated and detected predominantly in the 49 kDa band. Together with experiments involving treatment with\nphosphatases and Ser250 substitutions, the results indicate that the complex behaviour of Bcnt/Cfdp1 on SDS/PAGE\nis caused mainly by an acidic stretch in the N-terminal region and Ser250 phosphorylation in BCNT-C. Furthermore,\nBcnt/Cfdp1 is acetylated in vitro by CREB-binding protein (CBP) and four lysine residues including Lys268 in BCNT-C\nare also acetylated in vivo, revealing a protein regulated at multiple levels.", "subitem_description_type": "Abstract"}]}, "item_8_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "10.1042/BSR20150111 ", "subitem_relation_type_select": "DOI"}}]}, "item_8_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0144-8463", "subitem_source_identifier_type": "ISSN"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "metadata only access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_14cb"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Iwashita, Shintaro"}], "nameIdentifiers": [{"nameIdentifier": "472970", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Suzuki, Takehiro"}], "nameIdentifiers": [{"nameIdentifier": "472971", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "安田, 武嗣"}], "nameIdentifiers": [{"nameIdentifier": "472972", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nakashima, Kentaro"}], "nameIdentifiers": [{"nameIdentifier": "472973", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sakamoto, Taiichi"}], "nameIdentifiers": [{"nameIdentifier": "472974", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kohno, Toshiyuki"}], "nameIdentifiers": [{"nameIdentifier": "472975", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Takahashi, Ichiro"}], "nameIdentifiers": [{"nameIdentifier": "472976", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kobayashi, Takayasu"}], "nameIdentifiers": [{"nameIdentifier": "472977", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ohno-Iwashita, Yoshiko"}], "nameIdentifiers": [{"nameIdentifier": "472978", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Imajoh-Ohmi, Shinobu"}], "nameIdentifiers": [{"nameIdentifier": "472979", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Song, Si-Young"}], "nameIdentifiers": [{"nameIdentifier": "472980", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "安田 武嗣", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "472981", "nameIdentifierScheme": "WEKO"}]}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Mammalian Bcnt/Cfdp1, a potential epigenetic factor characterized by an acidic stretch in the disordered N-terminal and Ser250 phosphorylation in the conserved C-terminal regions", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Mammalian Bcnt/Cfdp1, a potential epigenetic factor characterized by an acidic stretch in the disordered N-terminal and Ser250 phosphorylation in the conserved C-terminal regions"}]}, "item_type_id": "8", "owner": "1", "path": ["1"], "permalink_uri": "https://repo.qst.go.jp/records/47288", "pubdate": {"attribute_name": "公開日", "attribute_value": "2015-09-15"}, "publish_date": "2015-09-15", "publish_status": "0", "recid": "47288", "relation": {}, "relation_version_is_last": true, "title": ["Mammalian Bcnt/Cfdp1, a potential epigenetic factor characterized by an acidic stretch in the disordered N-terminal and Ser250 phosphorylation in the conserved C-terminal regions"], "weko_shared_id": -1}
Mammalian Bcnt/Cfdp1, a potential epigenetic factor characterized by an acidic stretch in the disordered N-terminal and Ser250 phosphorylation in the conserved C-terminal regions
https://repo.qst.go.jp/records/47288
https://repo.qst.go.jp/records/47288016be903-480f-4210-bfb9-98559debc1d8
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2015-09-15 | |||||
タイトル | ||||||
タイトル | Mammalian Bcnt/Cfdp1, a potential epigenetic factor characterized by an acidic stretch in the disordered N-terminal and Ser250 phosphorylation in the conserved C-terminal regions | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
著者 |
Iwashita, Shintaro
× Iwashita, Shintaro× Suzuki, Takehiro× 安田, 武嗣× Nakashima, Kentaro× Sakamoto, Taiichi× Kohno, Toshiyuki× Takahashi, Ichiro× Kobayashi, Takayasu× Ohno-Iwashita, Yoshiko× Imajoh-Ohmi, Shinobu× Song, Si-Young× 安田 武嗣 |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The BCNT (Bucentaur) superfamily is classified by an uncharacteristic conserved sequence of ∼80 amino acids (aa) at the C-terminus, BCNT-C (the conserved C-terminal region of Bcnt/Cfdp1). Whereas the yeast Swc5 and Drosophila Yeti homologues play crucial roles in chromatin remodelling organization, mammalian Bcnt/Cfdp1 (craniofacial developmental protein 1) remains poorly understood. The protein, which lacks cysteine, is largely disordered and comprises an acidic N-terminal region, a lysine/glutamic acid/proline-rich 40 aa sequence and BCNT-C. It shows complex mobility on SDS/PAGE at ∼50 kDa, whereas its calculated molecular mass is ∼33 kDa. To characterize this mobility discrepancy and the effects of post-translational modifications (PTMs), we expressed various deleted His– Bcnt in E. coli and HEK cells and found that an acidic stretch in the N-terminal region is a main cause of the gel shift. Exogenous BCNT/CFDP1 constitutively expressed in HEK clones appears as a doublet at 49 and 47 kDa, slower than the protein expressed in Escherichia coli but faster than the endogenous protein on SDS/PAGE. Among seven in vivo phosphorylation sites, Ser250, which resides in a region between disordered and ordered regions in BCNT-C, is heavily phosphorylated and detected predominantly in the 49 kDa band. Together with experiments involving treatment with phosphatases and Ser250 substitutions, the results indicate that the complex behaviour of Bcnt/Cfdp1 on SDS/PAGE is caused mainly by an acidic stretch in the N-terminal region and Ser250 phosphorylation in BCNT-C. Furthermore, Bcnt/Cfdp1 is acetylated in vitro by CREB-binding protein (CBP) and four lysine residues including Lys268 in BCNT-C are also acetylated in vivo, revealing a protein regulated at multiple levels. |
|||||
書誌情報 |
Bioscience Reports 巻 35, 号 4, p. e00228, 発行日 2015-06 |
|||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0144-8463 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1042/BSR20150111 |