WEKO3
アイテム
{"_buckets": {"deposit": "4b58962a-e16c-4097-b4c1-87eed4c7142a"}, "_deposit": {"created_by": 1, "id": "47080", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "47080"}, "status": "published"}, "_oai": {"id": "oai:repo.qst.go.jp:00047080", "sets": ["1"]}, "author_link": ["470205", "470201", "470200", "470203", "470202", "470206", "470197", "470199", "470209", "470204", "470198", "470207", "470208"], "item_8_biblio_info_7": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2015-01", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "3", "bibliographicPageEnd": "1713", "bibliographicPageStart": "1700", "bibliographicVolumeNumber": "43", "bibliographic_titles": [{"bibliographic_title": "Nucleic Acids Research"}]}]}, "item_8_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "In mammalian nucleotide excision repair, the DDB1–\nDDB2 complex recognizes UV-induced DNA photolesions\nand facilitates recruitment of the XPC complex.\nUpon binding to damaged DNA, the Cullin 4\nubiquitin ligase associated with DDB1–DDB2 is activated\nand ubiquitinates DDB2 and XPC. The structurally\ndisordered N-terminal tail of DDB2 contains\nseven lysines identified as major sites for ubiquitination\nthat target the protein for proteasomal degradation;\nhowever, the precise biological functions of\nthese modifications remained unknown. By exogenous\nexpression of mutant DDB2 proteins in normal\nhuman fibroblasts, here we show that the N-terminal\ntail of DDB2 is involved in regulation of cellular responses\nto UV. By striking contrast with behaviors\nof exogenous DDB2, the endogenous DDB2 protein\nwas stabilized even after UV irradiation as a function\nof the XPC expression level. Furthermore, XPC competitively\nsuppressed ubiquitination of DDB2in vitro,\nand this effect was significantly promoted by centrin-\n2, which augments the DNA damage-recognition activity\nof XPC. Based on these findings, we propose\nthat in cells exposed to UV, DDB2 is protected by XPC\nfrom ubiquitination and degradation in a stochastic\nmanner; thus XPC allows DDB2 to initiate multiple\nrounds of repair events, thereby contributing to the\npersistence of cellular DNA repair capacity.", "subitem_description_type": "Abstract"}]}, "item_8_publisher_8": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Oxford Journals"}]}, "item_8_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "doi: 10.1093/nar/gkv038", "subitem_relation_type_select": "DOI"}}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "metadata only access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_14cb"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Matsumoto, Syota"}], "nameIdentifiers": [{"nameIdentifier": "470197", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "S., Fischer Eric"}], "nameIdentifiers": [{"nameIdentifier": "470198", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "安田, 武嗣"}], "nameIdentifiers": [{"nameIdentifier": "470199", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Dohmae, Naoshi"}], "nameIdentifiers": [{"nameIdentifier": "470200", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Iwai, Shigenori"}], "nameIdentifiers": [{"nameIdentifier": "470201", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Mori, Toshio"}], "nameIdentifiers": [{"nameIdentifier": "470202", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nishi, Ryotaro"}], "nameIdentifiers": [{"nameIdentifier": "470203", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yoshino, Ken-ichi"}], "nameIdentifiers": [{"nameIdentifier": "470204", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sakai, Wataru"}], "nameIdentifiers": [{"nameIdentifier": "470205", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hanaoka, Fumio"}], "nameIdentifiers": [{"nameIdentifier": "470206", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nicolas, H. Thom¨a"}], "nameIdentifiers": [{"nameIdentifier": "470207", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "al., et"}], "nameIdentifiers": [{"nameIdentifier": "470208", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "安田 武嗣", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "470209", "nameIdentifierScheme": "WEKO"}]}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Functional regulation of the DNA damage-recognition factor DDB2 by ubiquitination and interaction with xeroderma pigmentosum group C protein", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Functional regulation of the DNA damage-recognition factor DDB2 by ubiquitination and interaction with xeroderma pigmentosum group C protein"}]}, "item_type_id": "8", "owner": "1", "path": ["1"], "permalink_uri": "https://repo.qst.go.jp/records/47080", "pubdate": {"attribute_name": "公開日", "attribute_value": "2015-04-07"}, "publish_date": "2015-04-07", "publish_status": "0", "recid": "47080", "relation": {}, "relation_version_is_last": true, "title": ["Functional regulation of the DNA damage-recognition factor DDB2 by ubiquitination and interaction with xeroderma pigmentosum group C protein"], "weko_shared_id": -1}
Functional regulation of the DNA damage-recognition factor DDB2 by ubiquitination and interaction with xeroderma pigmentosum group C protein
https://repo.qst.go.jp/records/47080
https://repo.qst.go.jp/records/470800dba6abe-617e-4eea-8bce-0445ed3fbebb
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2015-04-07 | |||||
タイトル | ||||||
タイトル | Functional regulation of the DNA damage-recognition factor DDB2 by ubiquitination and interaction with xeroderma pigmentosum group C protein | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
著者 |
Matsumoto, Syota
× Matsumoto, Syota× S., Fischer Eric× 安田, 武嗣× Dohmae, Naoshi× Iwai, Shigenori× Mori, Toshio× Nishi, Ryotaro× Yoshino, Ken-ichi× Sakai, Wataru× Hanaoka, Fumio× Nicolas, H. Thom¨a× al., et× 安田 武嗣 |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | In mammalian nucleotide excision repair, the DDB1– DDB2 complex recognizes UV-induced DNA photolesions and facilitates recruitment of the XPC complex. Upon binding to damaged DNA, the Cullin 4 ubiquitin ligase associated with DDB1–DDB2 is activated and ubiquitinates DDB2 and XPC. The structurally disordered N-terminal tail of DDB2 contains seven lysines identified as major sites for ubiquitination that target the protein for proteasomal degradation; however, the precise biological functions of these modifications remained unknown. By exogenous expression of mutant DDB2 proteins in normal human fibroblasts, here we show that the N-terminal tail of DDB2 is involved in regulation of cellular responses to UV. By striking contrast with behaviors of exogenous DDB2, the endogenous DDB2 protein was stabilized even after UV irradiation as a function of the XPC expression level. Furthermore, XPC competitively suppressed ubiquitination of DDB2in vitro, and this effect was significantly promoted by centrin- 2, which augments the DNA damage-recognition activity of XPC. Based on these findings, we propose that in cells exposed to UV, DDB2 is protected by XPC from ubiquitination and degradation in a stochastic manner; thus XPC allows DDB2 to initiate multiple rounds of repair events, thereby contributing to the persistence of cellular DNA repair capacity. |
|||||
書誌情報 |
Nucleic Acids Research 巻 43, 号 3, p. 1700-1713, 発行日 2015-01 |
|||||
出版者 | ||||||
出版者 | Oxford Journals | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | doi: 10.1093/nar/gkv038 |