量研学術機関リポジトリ「QST-Repository」は、国立研究開発法人 量子科学技術研究開発機構に所属する職員等が生み出した学術成果（学会誌発表論文、学会発表、研究開発報告書、特許等）を集積しインターネット上で広く公開するサービスです。 Welcome to QST-Repository where we accumulates and discloses the academic research results(Journal Publications, Conference presentation, Research and Development Report, Patent, etc.) of the members of National Institutes for Quantum and Radiological Science and Technology.
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Sensitive and Rapid Detection of Centromeric Alphoid DNA in Human Metaphase Chromosomes by PNA Fluorescence In Situ Hybridization and Its Application to Biological Radiation Dosimetry
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Centromeres of human chromosomes contain highly repeated sequences of DNA including alphoid DNA. Because of the complicated genomic organization of the centromere, the distribution of alphoid DNA in chromosomes has not been fully investigated. We conducted fluorescence in situ hybridization using a synthetic peptide nucleic acid as a sensitive probe (PNA-FISH) to detect chromosomal sites of alphoid DNA. As a result, the size variation of centromeric alphoid DNA among chromosomes was visualized with hybridization times as short as 1 - 2 h. In addition to the inter-chromosomal variation, we detected possible inter-individual variation in the size of alphoid DNA sites, which had been difficult to precisely analyze by conventional molecular and cytogenetic methods. We then applied this sensitive and rapid detection method to evaluate the yield of multicentric chromosomes induced in cultured human peripheral blood lymphocytes by high-dose gamma-irradiation. This PNA-FISH allows us to unequivocally determine centromeres in complexly rearranged chromosomes, validating its usefulness in biological radiation dosimetry.
Sensitive and Rapid Detection of Centromeric Alphoid DNA in Human Metaphase Chromosomes by PNA Fluorescence In Situ Hybridization and Its Application to Biological Radiation Dosimetry
