WEKO3
アイテム
{"_buckets": {"deposit": "a694d3fc-5475-4505-abae-5b22572973e1"}, "_deposit": {"created_by": 1, "id": "46359", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "46359"}, "status": "published"}, "_oai": {"id": "oai:repo.qst.go.jp:00046359", "sets": ["1"]}, "author_link": ["461805", "461808", "461807", "461809", "461812", "461806", "461810", "461814", "461803", "461811", "461804", "461813"], "item_8_biblio_info_7": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2011-06", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "12", "bibliographicPageEnd": "1316", "bibliographicPageStart": "1309", "bibliographicVolumeNumber": "1810", "bibliographic_titles": [{"bibliographic_title": "Biochimica et Biophysica Acta. General Subjects"}]}]}, "item_8_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "BACKGROUND: Gd-DTPA-enhanced magnetic resonance imaging (MRI) is a conventional method for non-invasive investigation of blood-brain-barrier (BBB) permeability in animal models. It allows the visualization of serious injury to the BBB. We developed a novel approach for detecting very small disruptions in BBB permeability induced by dietary cholesterol by using carbamoyl-PROXYL (CMP) as an MRI contrast probe.\n\\nMETHODS: Mice were separated into two groups: normal diet (ND-mice) and high cholesterol diet (CD-mice). MRI-signal dynamics, plasma cholesterol, matrix metalloproteinase (MMP-9, MMP-2), and the white blood cell profile were analyzed. For the MRI analysis, two regions-of-interest (ROI) were selected: brain (ROI-1) and surrounding area (ROI-2).\n\\nRESULTS: In the ROI-2 of ND-mice, CMP- or Gd-enhanced MRI-signal followed typical kinetics with a half-life of signal decay (t(1/2)) ~8 or ~15min, respectively. In CD-mice, the MRI-signal increased continuously without decay. In the ROI-1 of ND- and CD-mice, MRI-signal enhancement was not detected by Gd-DTPA. In the ROI-1 of ND-mice, CMP-induced MRI-signal enhancement was negligible, while in CD-mice, it was significant (t(1/2)\u003e15min). Hypercholesterolemia increased the plasma levels of MMP-9 and neutrophils.\n\\nCONCLUSIONS: Hypercholesterolemia increases vascular permeability, which is mediated by MMP-9 and neutrophils.\n\\nGENERAL SIGNIFICANCE: Even very small disruptions in brain vascular permeability could be detected by CMP-enhanced MRI but not by Gd-DTPA-enhanced MRI.", "subitem_description_type": "Abstract"}]}, "item_8_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "10.1016/j.bbagen.2011.06.011", "subitem_relation_type_select": "DOI"}}]}, "item_8_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0304-4165", "subitem_source_identifier_type": "ISSN"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "metadata only access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_14cb"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Tomizawa, Atsuyuki"}], "nameIdentifiers": [{"nameIdentifier": "461803", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ishii, Itsuko"}], "nameIdentifiers": [{"nameIdentifier": "461804", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Zhelev, Zhivko"}], "nameIdentifiers": [{"nameIdentifier": "461805", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Aoki, Ichio"}], "nameIdentifiers": [{"nameIdentifier": "461806", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Shibata, Sayaka"}], "nameIdentifiers": [{"nameIdentifier": "461807", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Bakalova-Zheleva, Rumiana"}], "nameIdentifiers": [{"nameIdentifier": "461808", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kitada, Mitsukazu"}], "nameIdentifiers": [{"nameIdentifier": "461809", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "石井 伊都子", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "461810", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Zhelev Zhivko", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "461811", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "青木 伊知男", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "461812", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "柴田 さやか", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "461813", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "バカロバ ルミアナ", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "461814", "nameIdentifierScheme": "WEKO"}]}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Carbamoyl-PROXYL-enhanced MRI detects very small disruptions in brain vascular permeability induced by dietary cholesterol.", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Carbamoyl-PROXYL-enhanced MRI detects very small disruptions in brain vascular permeability induced by dietary cholesterol."}]}, "item_type_id": "8", "owner": "1", "path": ["1"], "permalink_uri": "https://repo.qst.go.jp/records/46359", "pubdate": {"attribute_name": "公開日", "attribute_value": "2012-07-13"}, "publish_date": "2012-07-13", "publish_status": "0", "recid": "46359", "relation": {}, "relation_version_is_last": true, "title": ["Carbamoyl-PROXYL-enhanced MRI detects very small disruptions in brain vascular permeability induced by dietary cholesterol."], "weko_shared_id": -1}
Carbamoyl-PROXYL-enhanced MRI detects very small disruptions in brain vascular permeability induced by dietary cholesterol.
https://repo.qst.go.jp/records/46359
https://repo.qst.go.jp/records/463595697615b-a411-4811-88f9-78bf8069d3d9
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2012-07-13 | |||||
タイトル | ||||||
タイトル | Carbamoyl-PROXYL-enhanced MRI detects very small disruptions in brain vascular permeability induced by dietary cholesterol. | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
著者 |
Tomizawa, Atsuyuki
× Tomizawa, Atsuyuki× Ishii, Itsuko× Zhelev, Zhivko× Aoki, Ichio× Shibata, Sayaka× Bakalova-Zheleva, Rumiana× Kitada, Mitsukazu× 石井 伊都子× Zhelev Zhivko× 青木 伊知男× 柴田 さやか× バカロバ ルミアナ |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | BACKGROUND: Gd-DTPA-enhanced magnetic resonance imaging (MRI) is a conventional method for non-invasive investigation of blood-brain-barrier (BBB) permeability in animal models. It allows the visualization of serious injury to the BBB. We developed a novel approach for detecting very small disruptions in BBB permeability induced by dietary cholesterol by using carbamoyl-PROXYL (CMP) as an MRI contrast probe. \nMETHODS: Mice were separated into two groups: normal diet (ND-mice) and high cholesterol diet (CD-mice). MRI-signal dynamics, plasma cholesterol, matrix metalloproteinase (MMP-9, MMP-2), and the white blood cell profile were analyzed. For the MRI analysis, two regions-of-interest (ROI) were selected: brain (ROI-1) and surrounding area (ROI-2). \nRESULTS: In the ROI-2 of ND-mice, CMP- or Gd-enhanced MRI-signal followed typical kinetics with a half-life of signal decay (t(1/2)) ~8 or ~15min, respectively. In CD-mice, the MRI-signal increased continuously without decay. In the ROI-1 of ND- and CD-mice, MRI-signal enhancement was not detected by Gd-DTPA. In the ROI-1 of ND-mice, CMP-induced MRI-signal enhancement was negligible, while in CD-mice, it was significant (t(1/2)>15min). Hypercholesterolemia increased the plasma levels of MMP-9 and neutrophils. \nCONCLUSIONS: Hypercholesterolemia increases vascular permeability, which is mediated by MMP-9 and neutrophils. \nGENERAL SIGNIFICANCE: Even very small disruptions in brain vascular permeability could be detected by CMP-enhanced MRI but not by Gd-DTPA-enhanced MRI. |
|||||
書誌情報 |
Biochimica et Biophysica Acta. General Subjects 巻 1810, 号 12, p. 1309-1316, 発行日 2011-06 |
|||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0304-4165 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.bbagen.2011.06.011 |