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In a mouse colon carcinoma (Colon-26) model, we have shown that 64Cu-ATSM preferentially localizes in intratumoral regions with a high density of CD133+ cells, which show characteristics of cancer stem cells or cancer stem cell-like cells (collectively referred here as CSCs). In this study, we evaluated the therapeutic effect of 64Cu-ATSM in relation to CD133 expression using this model.\n\\nMethods\nSystemic administration of 37 MBq 64Cu-ATSM or saline was conducted twice within a 1-week interval to mice bearing 1-week-old Colon-26 tumors (days 0\u0026#8211;7). At day 19, tumor size measurement, flow cytometry analysis and experimental lung metastatic assay were performed. The therapeutic effect of 64Cu-ATSM on sorted CD133+ and CD133\u0026#8722; Colon-26 cells was also examined in vitro.\n\\nResults\nIn vivo studies showed that 64Cu-ATSM treatment inhibited tumor growth. The percentage of CD133+ cells and metastatic ability in 64Cu-ATSM treated tumors was decreased compared with that in control animals. In vitro studies demonstrated that 64Cu-ATSM accumulated in cells under hypoxic conditions and incorporation of 64Cu-ATSM under hypoxia caused cell death in both CD133+ and CD133\u0026#8722; cells in a similar extent.\n\\nConclusions\n64Cu-ATSM administration reduced tumor volume as well as the percentage of CD133+ cells and the metastatic ability of Colon-26 tumors. Together with our data, it is suggested that 64Cu-ATSM accumulates in regions high in CD133+ highly tumorigenic cells and kills such regions by radiation, resulting in a decrease of the percentage of CD133+ cells.", "subitem_description_type": "Abstract"}]}, "item_8_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "10.1016/j.nucmedbio.2010.08.009", "subitem_relation_type_select": "DOI"}}]}, "item_8_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0969-8051", "subitem_source_identifier_type": "ISSN"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "metadata only access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_14cb"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Yoshii, Yukie"}], "nameIdentifiers": [{"nameIdentifier": "460378", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Furukawa, Takako"}], "nameIdentifiers": [{"nameIdentifier": "460379", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kiyono, Yashushi"}], "nameIdentifiers": [{"nameIdentifier": "460380", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Mori, Tetsuya"}], "nameIdentifiers": [{"nameIdentifier": "460381", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Watanabe, Ryo"}], "nameIdentifiers": [{"nameIdentifier": "460382", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yoshii, Hiroshi"}], "nameIdentifiers": [{"nameIdentifier": "460383", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Fujibayashi, Yasuhisa"}], "nameIdentifiers": [{"nameIdentifier": "460384", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "et.al"}], "nameIdentifiers": [{"nameIdentifier": "460385", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "吉井 幸恵", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "460386", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "古川 高子", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "460387", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "藤林 康久", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "460388", "nameIdentifierScheme": "WEKO"}]}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Internal radiotherapy with copper-64-diacetyl-bis (N4-methylthiosemicarbazone) reduces CD133+ highly tumorigenic cells and metastatic ability of mouse colon carcinoma", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Internal radiotherapy with copper-64-diacetyl-bis (N4-methylthiosemicarbazone) reduces CD133+ highly tumorigenic cells and metastatic ability of mouse colon carcinoma"}]}, "item_type_id": "8", "owner": "1", "path": ["1"], "permalink_uri": "https://repo.qst.go.jp/records/46231", "pubdate": {"attribute_name": "公開日", "attribute_value": "2011-12-21"}, "publish_date": "2011-12-21", "publish_status": "0", "recid": "46231", "relation": {}, "relation_version_is_last": true, "title": ["Internal radiotherapy with copper-64-diacetyl-bis (N4-methylthiosemicarbazone) reduces CD133+ highly tumorigenic cells and metastatic ability of mouse colon carcinoma"], "weko_shared_id": -1}
Internal radiotherapy with copper-64-diacetyl-bis (N4-methylthiosemicarbazone) reduces CD133+ highly tumorigenic cells and metastatic ability of mouse colon carcinoma
https://repo.qst.go.jp/records/46231
https://repo.qst.go.jp/records/462318e681699-0a9e-4143-a4c9-82ff22dab337
Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2011-12-21 | |||||
タイトル | ||||||
タイトル | Internal radiotherapy with copper-64-diacetyl-bis (N4-methylthiosemicarbazone) reduces CD133+ highly tumorigenic cells and metastatic ability of mouse colon carcinoma | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
著者 |
Yoshii, Yukie
× Yoshii, Yukie× Furukawa, Takako× Kiyono, Yashushi× Mori, Tetsuya× Watanabe, Ryo× Yoshii, Hiroshi× Fujibayashi, Yasuhisa× et.al× 吉井 幸恵× 古川 高子× 藤林 康久 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Introduction 64Cu-diacetyl-bis (N4-methylthiosemicarbazone) (64Cu-ATSM) is an imaging agent for positron emission tomography (PET) that targets hypoxic tumors. 64Cu-ATSM is also reported to be a potential agent for internal radiotherapy. In a mouse colon carcinoma (Colon-26) model, we have shown that 64Cu-ATSM preferentially localizes in intratumoral regions with a high density of CD133+ cells, which show characteristics of cancer stem cells or cancer stem cell-like cells (collectively referred here as CSCs). In this study, we evaluated the therapeutic effect of 64Cu-ATSM in relation to CD133 expression using this model. \nMethods Systemic administration of 37 MBq 64Cu-ATSM or saline was conducted twice within a 1-week interval to mice bearing 1-week-old Colon-26 tumors (days 0–7). At day 19, tumor size measurement, flow cytometry analysis and experimental lung metastatic assay were performed. The therapeutic effect of 64Cu-ATSM on sorted CD133+ and CD133− Colon-26 cells was also examined in vitro. \nResults In vivo studies showed that 64Cu-ATSM treatment inhibited tumor growth. The percentage of CD133+ cells and metastatic ability in 64Cu-ATSM treated tumors was decreased compared with that in control animals. In vitro studies demonstrated that 64Cu-ATSM accumulated in cells under hypoxic conditions and incorporation of 64Cu-ATSM under hypoxia caused cell death in both CD133+ and CD133− cells in a similar extent. \nConclusions 64Cu-ATSM administration reduced tumor volume as well as the percentage of CD133+ cells and the metastatic ability of Colon-26 tumors. Together with our data, it is suggested that 64Cu-ATSM accumulates in regions high in CD133+ highly tumorigenic cells and kills such regions by radiation, resulting in a decrease of the percentage of CD133+ cells. |
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書誌情報 |
Nuclear Medicine and Biology 巻 38, 号 2, p. 151-157, 発行日 2010-10 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0969-8051 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.nucmedbio.2010.08.009 |