WEKO3
アイテム
{"_buckets": {"deposit": "481331ac-5a4d-4de8-9e47-b17356fe1a69"}, "_deposit": {"created_by": 1, "id": "45760", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "45760"}, "status": "published"}, "_oai": {"id": "oai:repo.qst.go.jp:00045760", "sets": ["1"]}, "author_link": ["454857", "454867", "454873", "454870", "454863", "454866", "454875", "454865", "454872", "454869", "454856", "454859", "454861", "454874", "454862", "454868", "454860", "454871", "454864", "454858"], "item_8_biblio_info_7": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2010-06", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "6", "bibliographicPageEnd": "493", "bibliographicPageStart": "488", "bibliographicVolumeNumber": "64", "bibliographic_titles": [{"bibliographic_title": "Synapse"}]}]}, "item_8_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "In the central nervous system, translocator protein\n(TSPO) (18 kDa) (Papadopoulos et al., 2006), formerly\nnamed peripheral-type benzodiazepine receptor\n(PBR), is located in the microglial cells of the brain\n(Braestrup et al., 1977; Stephenson et al., 1995).\nMany studies have elucidated the relationship\nbetween TSPO and neurodegenerative diseases, such\nas Alzheimer\u0027s disease (Maeda et al., 2007; Yasuno\net al., 2008), and stroke-induced brain injury (Rojas\net al., 2007). To image TSPO in vivo, a number of\nnew ligands have been labeled with the positron emitters\n11C and 18F (Chauveau et al., 2008). We have\ndeveloped [11C]DAA1106 (Zhang et al., 2003; Maeda\net al., 2004), [18F]FEDAA1106 (Zhang et al., 2004),\nand [11C]AC-5216 (Zhang et al., 2007) for clinical\nimaging of TSPO in the brain. We recently synthesized\nand evaluated N-benzyl-N-[11C]methyl-2-(7-\nmethyl-8-oxo-2-phenyl-7, 8-dihydro-9H-purin-9-yl)acetamide\nwith a specific activity of 37\u0026#8211;110 GBq/lmol for\nthe imaging of TSPO (Yanamoto et al., 2009).\nThe aim of this study was to synthesize N-benzyl-\nN-methyl-2-(7-[11C]methyl-8-oxo-2-phenyl-7,8-dihydro-\n9H-purin-9-yl)acetamide ([11C]DAC) with ultra-high\nspecific activity (average 4560 GBq/lmol), and to\ncharacterize specific binding to TSPO in the rat brain\nafter ischemia. Using [11C]DAC of high specific activity,\nwe expected to acquire high uptake ratios\nbetween the ipsilateral and contralateral sides and\nhigher binding potential for TSPO from autoradiographic\nand PET images of infarct brains. Here, we\nlabeled the methyl group of purine ring of DAC to\nachieve ultra-high specific activity using [11C]CH3I\nthat was prepared by iodination of [11C]CH4 (Noguchi\nand Suzuki, 2003; Noguchi et al., 2008). We\nperformed comparative evaluation using [11C]DAC\nwith middle specific activity and low specific activity\nto elucidate the effect of specific activity on the in\nvitro and in vivo specific binding of [11C]DAC to\nTSPO in an infarct rat brain after ischemia. In this\narticle we use the terms \u0027\u0027high SA,\u0027\u0027 \u0027\u0027middle SA,\u0027\u0027 and\n\u0027\u0027low SA\u0027\u0027 to refer to specific activities of 3540\u0026#8211;5580,\n29\u0026#8211;52, and 0.3\u0026#8211;0.4 GBq/lmol, respectively.", "subitem_description_type": "Abstract"}]}, "item_8_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0887-4476", "subitem_source_identifier_type": "ISSN"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "metadata only access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_14cb"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Yui, Joji"}], "nameIdentifiers": [{"nameIdentifier": "454856", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hatori, Akiko"}], "nameIdentifiers": [{"nameIdentifier": "454857", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yanamoto, Kazuhiko"}], "nameIdentifiers": [{"nameIdentifier": "454858", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Takei, Makoto"}], "nameIdentifiers": [{"nameIdentifier": "454859", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nengaki, Nobuki"}], "nameIdentifiers": [{"nameIdentifier": "454860", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kumata, Katsushi"}], "nameIdentifiers": [{"nameIdentifier": "454861", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kawamura, Kazunori"}], "nameIdentifiers": [{"nameIdentifier": "454862", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yamasaki, Tomoteru"}], "nameIdentifiers": [{"nameIdentifier": "454863", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Suzuki, Kazutoshi"}], "nameIdentifiers": [{"nameIdentifier": "454864", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Zhang, Ming-Rong"}], "nameIdentifiers": [{"nameIdentifier": "454865", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "由井 譲二", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "454866", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "羽鳥 晶子", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "454867", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "柳本 和彦", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "454868", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "武井 誠", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "454869", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "念垣 信樹", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "454870", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "熊田 勝志", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "454871", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "河村 和紀", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "454872", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "山崎 友照", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "454873", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "鈴木 和年", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "454874", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "張 明栄", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "454875", "nameIdentifierScheme": "WEKO"}]}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Imaging of the translocator protein (18kDa) in rat brain after ischemia using [11C]DAC with ultra-high specific Activity", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Imaging of the translocator protein (18kDa) in rat brain after ischemia using [11C]DAC with ultra-high specific Activity"}]}, "item_type_id": "8", "owner": "1", "path": ["1"], "permalink_uri": "https://repo.qst.go.jp/records/45760", "pubdate": {"attribute_name": "公開日", "attribute_value": "2010-03-03"}, "publish_date": "2010-03-03", "publish_status": "0", "recid": "45760", "relation": {}, "relation_version_is_last": true, "title": ["Imaging of the translocator protein (18kDa) in rat brain after ischemia using [11C]DAC with ultra-high specific Activity"], "weko_shared_id": -1}
Imaging of the translocator protein (18kDa) in rat brain after ischemia using [11C]DAC with ultra-high specific Activity
https://repo.qst.go.jp/records/45760
https://repo.qst.go.jp/records/45760ad06f57a-fce1-422b-98d3-b9eba3f6ca01
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2010-03-03 | |||||
タイトル | ||||||
タイトル | Imaging of the translocator protein (18kDa) in rat brain after ischemia using [11C]DAC with ultra-high specific Activity | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
著者 |
Yui, Joji
× Yui, Joji× Hatori, Akiko× Yanamoto, Kazuhiko× Takei, Makoto× Nengaki, Nobuki× Kumata, Katsushi× Kawamura, Kazunori× Yamasaki, Tomoteru× Suzuki, Kazutoshi× Zhang, Ming-Rong× 由井 譲二× 羽鳥 晶子× 柳本 和彦× 武井 誠× 念垣 信樹× 熊田 勝志× 河村 和紀× 山崎 友照× 鈴木 和年× 張 明栄 |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | In the central nervous system, translocator protein (TSPO) (18 kDa) (Papadopoulos et al., 2006), formerly named peripheral-type benzodiazepine receptor (PBR), is located in the microglial cells of the brain (Braestrup et al., 1977; Stephenson et al., 1995). Many studies have elucidated the relationship between TSPO and neurodegenerative diseases, such as Alzheimer's disease (Maeda et al., 2007; Yasuno et al., 2008), and stroke-induced brain injury (Rojas et al., 2007). To image TSPO in vivo, a number of new ligands have been labeled with the positron emitters 11C and 18F (Chauveau et al., 2008). We have developed [11C]DAA1106 (Zhang et al., 2003; Maeda et al., 2004), [18F]FEDAA1106 (Zhang et al., 2004), and [11C]AC-5216 (Zhang et al., 2007) for clinical imaging of TSPO in the brain. We recently synthesized and evaluated N-benzyl-N-[11C]methyl-2-(7- methyl-8-oxo-2-phenyl-7, 8-dihydro-9H-purin-9-yl)acetamide with a specific activity of 37–110 GBq/lmol for the imaging of TSPO (Yanamoto et al., 2009). The aim of this study was to synthesize N-benzyl- N-methyl-2-(7-[11C]methyl-8-oxo-2-phenyl-7,8-dihydro- 9H-purin-9-yl)acetamide ([11C]DAC) with ultra-high specific activity (average 4560 GBq/lmol), and to characterize specific binding to TSPO in the rat brain after ischemia. Using [11C]DAC of high specific activity, we expected to acquire high uptake ratios between the ipsilateral and contralateral sides and higher binding potential for TSPO from autoradiographic and PET images of infarct brains. Here, we labeled the methyl group of purine ring of DAC to achieve ultra-high specific activity using [11C]CH3I that was prepared by iodination of [11C]CH4 (Noguchi and Suzuki, 2003; Noguchi et al., 2008). We performed comparative evaluation using [11C]DAC with middle specific activity and low specific activity to elucidate the effect of specific activity on the in vitro and in vivo specific binding of [11C]DAC to TSPO in an infarct rat brain after ischemia. In this article we use the terms ''high SA,'' ''middle SA,'' and ''low SA'' to refer to specific activities of 3540–5580, 29–52, and 0.3–0.4 GBq/lmol, respectively. |
|||||
書誌情報 |
Synapse 巻 64, 号 6, p. 488-493, 発行日 2010-06 |
|||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0887-4476 |