量研学術機関リポジトリ「QST-Repository」は、国立研究開発法人 量子科学技術研究開発機構に所属する職員等が生み出した学術成果（学会誌発表論文、学会発表、研究開発報告書、特許等）を集積しインターネット上で広く公開するサービスです。 Welcome to QST-Repository where we accumulates and discloses the academic research results(Journal Publications, Conference presentation, Research and Development Report, Patent, etc.) of the members of National Institutes for Quantum and Radiological Science and Technology.
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To study the transcriptome of embryonic stem cells,we used a new gene expression profiling meethod which can measure the expression levels of unknown and rarely expressed transcripts precisely.We detected a total of 33,136 signal peaks representing transcripts in E14 mouse embryonic stem cells.Subsequent random cloning of the peaks suggests that mouse embryonic stem cells express at least 40,000 transcripts,of which about 2,000 are still unknown.In addition,we identified 1,022 non-coding transcripts,several of which change depending on differentiation in gene expression.Our database provides a high-resolution expression profile of E14 cells and is applicable to other mouse ES cell analyses.It includes most transcription regulation-encoding genes and a significant number of unknown and non-coding transcripts.
More then 40,000 transcripts including novel and noncoding transcripts in mouse embryonic stem cells
