量研学術機関リポジトリ「QST-Repository」は、国立研究開発法人 量子科学技術研究開発機構に所属する職員等が生み出した学術成果（学会誌発表論文、学会発表、研究開発報告書、特許等）を集積しインターネット上で広く公開するサービスです。 Welcome to QST-Repository where we accumulates and discloses the academic research results(Journal Publications, Conference presentation, Research and Development Report, Patent, etc.) of the members of National Institutes for Quantum and Radiological Science and Technology.
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Recent improvements in DNA microarray technologies and bioinformatics have made it possible to look for common features of ionizing radiation (IR)-responsive genes and their regulatory regions. We analyzed the promoters of 217 IR- responsive human genes, compiled from microarray databases available in the literature. Using the DBTSS database, the transcriptional start sites were determined, and the core promoter elements, such as the TATA-box, initiator (Inr), GC- box and CCAAT-box, were searched for in the -1000bp/+200bp region of each gene by using MATCH. It was found
that the frequency of Inr in IR-responsive genes was higher than that in general genes, and the frequency of GC-box and CCAAT-box was significantly lower than that in general genes. Utilization of the GC-box and the CCAAT-box in IR-responsive genes was found to be dependent on the TATA-box status, that is, GC-box frequency was low in TATA box-containing genes, and CCAAT-box frequency was also low in TATA-less genes. When correlations between gene functions and frequencies of core promoter elements were examined, no apparent biased use of the core promoter element in association with a specific gene function was observed. It may be speculated that utilization of Inr in the core promoter correlates with appearance of IR-responsive enhancer(silencer) elements in the upstream (downstream) regulatory region.
The initiator motif is preferentially used as the core promoter element in ionizing radiation-responsive genes
